Affiliation:
1. Cardiovascular Genetics Center, Biomedical Research Institute of Girona, 17190 Salt, Spain
2. Department of Medical Sciences, Universitat de Girona, 17003 Girona, Spain
3. Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), 21005 Madrid, Spain
4. Hospital Josep Trueta, 17007 Girona, Spain
Abstract
Arrhythmogenic cardiomyopathy (ACM) is an inherited cardiac disease characterized by fibrofatty replacement of the myocardium. Deleterious variants in desmosomal genes are the main cause of ACM and lead to common and gene-specific molecular alterations, which are not yet fully understood. This article presents the first systematic in vitro study describing gene and protein expression alterations in desmosomes, electrical conduction-related genes, and genes involved in fibrosis and adipogenesis. Moreover, molecular and functional alterations in calcium handling were also characterized. This study was performed d with HL1 cells with homozygous knockouts of three of the most frequently mutated desmosomal genes in ACM: PKP2, DSG2, and DSC2 (generated by CRISPR/Cas9). Moreover, knockout and N-truncated clones of DSP were also included. Our results showed functional alterations in calcium handling, a slower calcium re-uptake was observed in the absence of PKP2, DSG2, and DSC2, and the DSP knockout clone showed a more rapid re-uptake. We propose that the described functional alterations of the calcium handling genes may be explained by mRNA expression levels of ANK2, CASQ2, ATP2A2, RYR2, and PLN. In conclusion, the loss of desmosomal genes provokes alterations in calcium handling, potentially contributing to the development of arrhythmogenic events in ACM.
Funder
Obra Social “La Caixa Foundation”
Fondo de Investigación Sanitaria
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference56 articles.
1. Arrhythmogenic Cardiomyopathy;Corrado;Circ. Res.,2017
2. Arrhythmogenic Left Ventricular Cardiomyopathy: A Clinical and CMR Study;He;Sci. Rep.,2020
3. Arrhythmogenic Right Ventricular Dysplasia: A United States Experience;Dalal;Heart Rhythm,2005
4. Arrhythmogenic Cardiomyopathy: Pathology, Genetics, and Concepts in Pathogenesis;Hoorntje;Cardiovasc. Res.,2017
5. Mattesi, G., Zorzi, A., Corrado, D., and Cipriani, A. (2020). Natural History of Arrhythmogenic Cardiomyopathy. J. Clin. Med., 9.
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