Transcriptomic Analysis of Flowering Time Genes in Cultivated Chickpea and Wild Cicer

Author:

Gretsova Maria1,Surkova Svetlana1ORCID,Kanapin Alexander2,Samsonova Anastasia2,Logacheva Maria3,Shcherbakov Andrey4,Logachev Anton1,Bankin Mikhail1ORCID,Nuzhdin Sergey5,Samsonova Maria1

Affiliation:

1. Mathematical Biology and Bioinformatics Laboratory, Peter the Great St. Petersburg Polytechnic University, 195251 St. Petersburg, Russia

2. Centre for Computational Biology, Peter the Great St. Petersburg Polytechnic University, 195251 St. Petersburg, Russia

3. Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia

4. Laboratory of Microbial Technology, All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia

5. Section of Molecular and Computational Biology, University of Southern California, Los Angeles, CA 90089, USA

Abstract

Chickpea (Cicer arietinum L.) is a major grain legume and a good source of plant-based protein. However, comprehensive knowledge of flowering time control in Cicer is lacking. In this study, we acquire high-throughput transcriptome sequencing data and analyze changes in gene expression during floral transition in the early flowering cultivar ICCV 96029, later flowering C. arietinum accessions, and two wild species, C. reticulatum and C. echinospermum. We identify Cicer orthologs of A. thaliana flowering time genes and analyze differential expression of 278 genes between four species/accessions, three tissue types, and two conditions. Our results show that the differences in gene expression between ICCV 96029 and other cultivated chickpea accessions are vernalization-dependent. In addition, we highlight the role of FTa3, an ortholog of FLOWERING LOCUS T in Arabidopsis, in the vernalization response of cultivated chickpea. A common set of differentially expressed genes was found for all comparisons between wild species and cultivars. The direction of expression change for different copies of the FT-INTERACTING PROTEIN 1 gene was variable in different comparisons, which suggests complex mechanisms of FT protein transport. Our study makes a contribution to the understanding of flowering time control in Cicer, and can provide genetic strategies to further improve this important agronomic trait.

Funder

Ministry of Science and Higher Education of the Russian Federation

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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