Lensed Fiber-Optic Two-Photon Endomicroscopy for Field-of-View Enhancement

Author:

Wang Conghao1,Liu Huilan12,Ma Jianrui1,Fu Qiang3,Li Yijun3,Chen Yanchuan3,Gao Yuqian3,Tian Jingquan3,Luo Xinlei3,Yu Fei45ORCID,Zhao Chunzhu6,Wu Runlong6,Wang Aimin7,Feng Lishuang128

Affiliation:

1. School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100191, China

2. Key Laboratory of Precision Opto-Mechatronics Technology (Ministry of Education), Beijing 100191, China

3. Beijing Transcend Vivoscope Biotech Co., Ltd., Beijing 100049, China

4. Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China

5. Key Laboratory of Materials for High Power Laser, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China

6. National Biomedical Imaging Center, State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing 100871, China

7. State Key Laboratory of Advanced Optical Communication System and Networks, School of Electronics, Peking University, Beijing 100871, China

8. Laboratory of Intelligent Sensing Materials and Chip Integration Technology of Zhejiang Province, Hangzhou Innovation Institute of Beihang University, Hangzhou 310063, China

Abstract

Two-photon endomicroscopy is a promising technique with the ability to achieve in situ imaging and diagnosis at subcellular resolution. The large field-of-view capability is essential and useful to locate and image suspicious areas of biological tissue. In this work, we report objective-lens-free, lensed fiber-optic two-photon endomicroscopy for field-of-view enhancement. The field of view of this two-photon endomicroscopic probe is 750 μm with a resolution of 3.03 μm. This 1.6 g miniature probe has an integrated outer diameter of 5.8 mm and a rigid length of 33.5 mm. The imaging performance of the lensed-fiber-optic two-photon endomicroscopy was validated by examining an ex vivo mouse heart, kidney, brain, stomach wall tissues, and in vivo brain tissue.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Publisher

MDPI AG

Subject

Radiology, Nuclear Medicine and imaging,Instrumentation,Atomic and Molecular Physics, and Optics

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Modeling and measurement of a miniature piezoelectric ceramic tube fiber scanner for two-photon endomicroscopy;Journal of Optical Microsystems;2024-01-13

2. Lensed fiber-optic scanning two-photon endomicroscopic imaging;Second Conference on Biomedical Photonics and Cross-Fusion (BPC 2023);2023-07-28

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3