Extensible LED-Induced Integrated Fluorescence Detection Module for Quantitative Analysis of Lucigenin Concentration

Author:

Qi Xiaoguang1ORCID,Hao Xianglong1,Zhang Muzi1,Jiang Lili1,Gao Wenyue1,Wu Chi123

Affiliation:

1. Shandong Provincial Center for In-Situ Marine Sensors, Institute of Marine Science and Technology, Shandong University, Qingdao 266237, China

2. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China

3. Shandong Provincial Center for In-Situ Marine Sensors, Aixsensor Co., Dezhou 253000, China

Abstract

We developed an extensible LED-induced fluorescence detection module with a highly integrated and ultra-compact structure. A target-oriented design methodology was used to demonstrate the module’s optimal design. Lucigenin solution was used as a test sample in evaluation trials to demonstrate the module’s quantitative fluorescence detection capability. Results showed that the integrated module has an outstanding linear response in the range of 0–1 μmol·L−1, with sensitivity and limit of detection (LOD) of 0.1692 V/μmol·L−1 and 0.03 μmol·L−1, respectively. Statistical analyses showed that our integrated module has extremely high repeatability and accuracy, i.e., the values of Pearson’s correlation coefficient and root-mean-square error exceeded 0.9995 and 1.8‰, respectively. More importantly, the integrated module possesses favorable extensibility and can realize on-demand rapid fluorescence-signal detection of other targets using appropriate parameter combinations. This module offers new opportunities for reliable, cost-effective and easy-to-use fluorescence-signal detection, especially in resource-constrained fluorescence detection applications.

Funder

Shandong Provincial Natural Science Foundation

Postdoctoral Innovation Project of Shandong Province

Qingdao Postdoctoral Applied Research Project

National Natural Science Foundation of China

Outstanding Youth Science Foundation of Shandong Province

Southern Marine Science and Engineering Guangdong Laboratory

Shandong University Foundation for Future Scholar Plan

Publisher

MDPI AG

Subject

Radiology, Nuclear Medicine and imaging,Instrumentation,Atomic and Molecular Physics, and Optics

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