Affiliation:
1. Department of Light Sources and Illuminating Engineering, Fudan University, 2005th Songhu Road, Shanghai 200441, China
2. Institute of Future Lighting, Academy for Engineering and Technology, Fudan University, 220th Handan Road, Shanghai 200433, China
3. Zhongshan Fudan Joint Innovation Center, 6th Xiangxing Road, Zhongshan 528403, China
Abstract
Transcranial photobiomodulation (tPBM) is an innovative intervention for a wide range of neurological and psychological conditions. tPBM therapy can enhance the metabolic capacity of neurons and bring about a variety of beneficial changes. This study mainly investigated the photobiological effects of pulsed red and near-infrared (NIR) light on neuron-like neuroblastoma SH-SY5Y cells by in vitro experiments. We covered the irradiation parameters, including wavelength (660, 850 nm), power density (5, 10, 20, 50, 100 mW/cm2), frequency (40, 100, 1000 Hz), and duty cycle (10%, 50%, 90%), finding that pulsed light generated a distinct effect compared with continuous-wave light on the cellular responses. Cell viability, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP), and reactive oxygen species (ROS) showed significant increase after irradiation of the adequate fluence amount (4.8–9.6 J/cm2), and the enhancement was more notable under 40 Hz pulsed lighting. Under pulsed lighting with an average power density of 10 mW/cm2, cells that received irradiation of higher peak power density up to 100 mW/cm2 with a 10% duty cycle showed slightly higher metabolic responses. In addition, it was found that under same total fluence, short-term irradiation with high power density was more effective than long-term irradiation with low power density, which indicated the existence of a threshold to achieve effectiveness.
Funder
the Development of Various Medical Instruments Based on Pulsed Light program funded by the Zhongshan Municipal Government
Subject
Radiology, Nuclear Medicine and imaging,Instrumentation,Atomic and Molecular Physics, and Optics
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