Puerarin Prevents Cadmium-Induced Neuronal Injury by Alleviating Autophagic Dysfunction in Rat Cerebral Cortical Neurons
-
Published:2023-05-05
Issue:9
Volume:24
Page:8328
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Wang Li12, Wang Tao12, Wen Shuangquan12, Song Ruilong12, Zou Hui12, Gu Jianhong12, Liu Xuezhong12, Bian Jianchun12ORCID, Liu Zongping12ORCID, Yuan Yan12ORCID
Affiliation:
1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China 2. Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
Abstract
Autophagic dysfunction is one of the main mechanisms of cadmium (Cd)-induced neurotoxicity. Puerarin (Pue) is a natural antioxidant extracted from the medicinal and edible homologous plant Pueraria lobata. Studies have shown that Pue has neuroprotective effects in a variety of brain injuries, including Cd-induced neuronal injury. However, the role of Pue in the regulation of autophagy to alleviate Cd-induced injury in rat cerebral cortical neurons remains unclear. This study aimed to elucidate the protective mechanism of Pue in alleviating Cd-induced injury in rat cerebral cortical neurons by targeting autophagy. Our results showed that Pue alleviated Cd-induced injury in rat cerebral cortical neurons in vitro and in vivo. Pue activates autophagy and alleviates Cd-induced autophagic blockade in rat cerebral cortical neurons. Further studies have shown that Pue alleviates the Cd-induced inhibition of autophagosome–lysosome fusion, as well as the inhibition of lysosomal degradation. The specific mechanism is related to Pue alleviating the inhibition of Cd on the expression levels of the key proteins Rab7, VPS41, and SNAP29, which regulate autophagosome–lysosome fusion, as well as the lysosome-related proteins LAMP2, CTSB, and CTSD. In summary, these results indicate that Pue alleviates Cd-induced autophagic dysfunction in rat cerebral cortical neurons by alleviating autophagosome–lysosome fusion dysfunction and lysosomal degradation dysfunction, thereby alleviating Cd-induced neuronal injury.
Funder
National Natural Science Foundation of China Natural Science Foundation of Jiangsu Province Jiangsu Higher Education Institutions
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference65 articles.
1. Cadmium exposure in living organisms: A short review;Zhang;Sci. Total Environ.,2019 2. A review on Cadmium Exposure in the Population and Intervention Strategies Against Cadmium Toxicity;Wang;Bull. Environ. Contam. Toxicol.,2021 3. Wen, S.Q., Wang, L., Zou, H., Gu, J.H., Song, R.L., Bian, J.C., Yuan, Y., and Liu, Z.P. (2021). Puerarin Attenuates Cadmium-Induced Neuronal Injury via Stimulating Cadmium Excretion, Inhibiting Oxidative Stress and Apoptosis. Biomolecules, 11. 4. Baicalein ameliorates cadmium-induced hepatic and renal oxidative damage in rats;Wang;Indian J. Anim. Res.,2019 5. Molybdenum and cadmium co-induce oxidative stress and apoptosis through mitochondria-mediated pathway in duck renal tubular epithelial cells;Wang;J. Hazard. Mater.,2020
|
|