Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes-Reference-Cited by-同舟云学术

Polymorphism of Saccharomyces cerevisiae Strains in DNA Metabolism Genes

Published:2023-04-25 Issue:9 Volume:24 Page:7795
ISSN:1422-0067
Container-title:International Journal of Molecular Sciences
language:en
Short-container-title:IJMS

Author:

Zhuk Anna S.¹²³^ORCID,Lada Artem G.⁴,Pavlov Youri I.⁵⁶^ORCID

Affiliation:

1. Institute of Applied Computer Science, ITMO University, 191002 St. Petersburg, Russia

2. Vavilov Institute of General Genetics, St. Petersburg Branch, Russian Academy of Sciences, 199034 St. Petersburg, Russia

3. Laboratory of Amyloid Biology, St. Petersburg State University, 199034 St. Petersburg, Russia

4. Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA

5. Eppley Institute for Research in Cancer, Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA

6. Departments of Biochemistry and Molecular Biology, Microbiology and Pathology, Genetics Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE 68198, USA

Abstract

Baker’s yeast, S. cerevisiae, is an excellent model organism exploited for molecular genetic studies of the mechanisms of genome stability in eukaryotes. Genetic peculiarities of commonly used yeast strains impact the processes of DNA replication, repair, and recombination (RRR). We compared the genomic DNA sequence variation of the five strains that are intensively used for RRR studies. We used yeast next-generation sequencing data to detect the extent and significance of variation in 183 RRR genes. We present a detailed analysis of the differences that were found even in closely related strains. Polymorphisms of common yeast strains should be considered when interpreting the outcomes of genome stability studies, especially in cases of discrepancies between laboratories describing the same phenomena.

Funder

NE DHHS

Fred and Pamela Buffett Cancer Center’s National Cancer Institute Cancer Support

Saint-Petersburg State University

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Link

https://www.mdpi.com/1422-0067/24/9/7795/pdf

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