A SiPM-Enabled Portable Delayed Fluorescence Photon Counting Device: Climatic Plant Stress Biosensing

Abstract

A portable and sensitive time-resolved biosensor for capturing very low intensity light emission is a promising avenue to study plant delayed fluorescence. These weak emissions provide insight on plant health and can be useful in plant science as well as in the development of accurate feedback indicators for plant growth and yield in applications of agricultural crop cultivation. A field-based delayed fluorescence device is also desirable to enable monitoring of plant stress response to climate change. Among basic techniques for the detection of rapidly fluctuating low intensity light is photon counting. Despite its vast utility, photon counting techniques often relying on photomultiplier tube (PMT) technology, having restricted use in agricultural and environment measurements of plant stress outside of the laboratory setting, mainly due to the prohibitive cost of the equipment, high voltage nature, and the complexity of its operation. However, recent development of the new generation solid-state silicon photomultiplier (SiPM) single photon avalanche diode array has enabled the availability of high quantum efficiency, easy-to-operate, compact, photon counting systems which are not constrained to sophisticated laboratories, and are accessible owing to their low-cost. In this contribution, we have conceived, fabricated and validated a novel SiPM-based photon counting device with integrated plug-and-play excitation LED, all housed inside a miniaturized sample chamber to record weak delayed fluorescence lifetime response from plant leaves subjected to varying temperature condition and drought stress. Findings from our device show that delayed fluorescence reports on the inactivation to the plant’s photosystem II function in response to unfavorable acute environmental heat and cold shock stress as well as chronic water deprivation. Results from our proof-of-concept miniaturized prototype demonstrate a new, simple and effective photon counting instrument is achieved, one which can be deployed in-field to rapidly and minimally invasively assess plant physiological growth and health based on rapid, ultra-weak delayed fluorescence measurements directly from a plant leaf.