Affiliation:
1. Department of Agriculture, Forestry and Bioresources, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea
2. Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong-gun 27709, Republic of Korea
3. Department of Medicinal Biosciences, Research Institute for Biomedicinal & Health Science, College of Biomedicinal and Health Science, Konkuk University, Chungju 27478, Republic of Korea
Abstract
Light plays vital roles in fungal growth, development, reproduction, and pigmentation. In Flammulina velutipes, the color of the fruiting body exhibits distinct changes in response to light; however, the underlying molecular mechanisms remain unknown. Therefore, in this study, we aimed to analyze the F. velutipes transcriptome under red, green, and blue light-emitting diode (LED) lights to identify the key genes affecting the light response and fruiting body color in this fungus. Additionally, we conducted protein–protein interaction (PPI) network analysis of the previously reported fruiting body color-related gene, Fvpal1, to identify the hub genes. Phenotypic analysis revealed that fruiting bodies exposed to green and blue lights were darker than those untreated or exposed to red light, with the color intensifying more after 48 h of exposure to blue light compared to that after 24 h of exposure. Differentially expressed gene (DEG) analyses of all light treatments for 24 h revealed that the numbers of DEGs were 17, 74, and 257 under red, green, and blue lights, respectively. Subsequently, functional enrichment analysis was conducted of the DEGs identified under green and blue lights, which influenced the color of F. velutipes. In total, 103 of 168 downregulated DEGs under blue and green lights were included in the enrichment analysis. Among the DEGs enriched under both green and blue light treatments, four genes were related to monooxygenases, with three genes annotated as cytochrome P450s that are crucial for various metabolic processes in fungi. PPI network analysis of Fvpal1 revealed associations with 11 genes, among which the expression of one gene, pyridoxal-dependent decarboxylase, was upregulated in F. velutipes exposed to blue light. These findings contribute to our understanding of the molecular mechanisms involved in the fruiting body color changes in response to light and offer potential molecular markers for further exploration of light-mediated regulatory pathways.
Funder
Rural Development Administration
Reference64 articles.
1. Phylogeny and species delimitation of Flammulina: Taxonomic status of winter mushroom in East Asia and a new European species identified using an integrated approach;Wang;Mycol. Prog.,2018
2. Chaisaena, W. (2009). Light Effects on Fruiting Body Development of Wildtype in Comparison to Light-Insensitive Mutant Strains of the Basidiomycete Coprinopsis cinerea, Grazing of Mites (Tyrophagus putrescentiae) on the Strains and Production of Volatile Organic Compounds during Fruiting Body Development. [Ph.D. Thesis, Georg-August-University].
3. Influences of light regimes on reproduction, germination, pigmentation, pathogenesis and overall development of a variety of Filamentous Fungi—A Review;Nmom;Asian J. Biol.,2021
4. Bayram, Ö.S., and Bayram, Ö. (2023). An anatomy of fungal eye: Fungal photoreceptors and signaling mechanisms. J. Fungi, 9.
5. Effects of different light quality of LED light source on growth rate, mycelium branch and biomass of straw mushroom mycelium;Hou;Acta Agric. Zhejiangensis,2021