Abstract
Aurantiochytrium is a heterotrophic marine microalga that has potential industrial applications. The main objectives of this study were to isolate an Aurantiochytrium strain from Sand Cay (Son Ca) Island, Vietnam, optimize its culture conditions, determine its nutritional composition, extract polyunsaturated fatty acids (PUFAs) in the free (FFA) and the alkyl ester (FAAE) forms, and evaluate the antioxidation and neuroprotection properties of the PUFAs. Aurantiochytrium sp. SC145 can be grown stably under laboratory conditions. Its culture conditions were optimized for a dry cell weight (DCW) of 31.18 g/L, with total lipids comprising 25.29%, proteins 7.93%, carbohydrates 15.21%, and carotenoid at 143.67 µg/L of DCW. The FAAEs and FFAs extracted from Aurantiochytrium sp. SC145 were rich in omega 3–6–9 fatty acids (40.73% and 44.00% of total fatty acids, respectively). No acute or subchronic oral toxicity was determined in mice fed with the PUFAs in FFA or FAAE forms at different doses over 90 days. Furthermore, the PUFAs in the FFA or FAAE forms and their main constituents of EPA, DHA, and ALA showed antioxidant and AChE inhibitory properties and neuroprotective activities against damage caused by H2O2- and amyloid-ß protein fragment 25–35 (Aβ25-35)-induced C6 cells. These data suggest that PUFAs extracted from Aurantiochytrium sp. SC145 may be a potential therapeutic target for the treatment of neurodegenerative disorders.
Funder
Joint Vietnam–Russia Tropical Science and Technology Research Center
Vietnam Academy of Science and Technology
Subject
Drug Discovery,Pharmacology, Toxicology and Pharmaceutics (miscellaneous),Pharmaceutical Science
Reference82 articles.
1. Valorisation of algal biomass to value-added metabolites: Emerging trends and opportunities;Uma;Phytochem. Rev.,2022
2. Dang, D.H., and Hoang, T.L.A. (2016). New Heterotrophic marine microalgae of Labyrinthula, Schizochytrium, Thraustochytrium in Vietnam: Potential and Challenges, Publisher of Science and Technology. (In Vietnamese).
3. Kumar, A., Singh, R.P., Kumar, I., Yadav, P., Singh, S.K., Singh, P.K., Gupta, R.K., Singh, S.M., and Kesawat, M.S. (2022). Algal Metabolites can be an immune Booster against COVID-19 Pandemic. Antioxidants, 11.
4. Maternal plasma DHA levels increase prior to 29 days post-lh surge in women undergoing frozen embryo transfer: A prospective, observational study of human pregnancy;Meyer;J. Clin. Endocrinol. Metab.,2016
5. Does Consumption of LC Omega-3 PUFA Enhance Cognitive Performance in Healthy School-Aged Children and throughout Adulthood? Evidence from Clinical Trials;Stonehouse;Nutrients,2014
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献