Advances in the Study of Common and Rare CFTR Complex Alleles Using Intestinal Organoids

Author:

Krasnova Maria1,Efremova Anna1,Mokrousova Diana1,Bukharova Tatiana1,Kashirskaya Nataliya12ORCID,Kutsev Sergey1,Kondratyeva Elena1,Goldshtein Dmitry1

Affiliation:

1. Research Centre for Medical Genetics, Moscow 115522, Russia

2. Moscow Regional Research and Clinical Institute (“MONIKI”), Schepkina Street, 61/2, 1, Moscow 129110, Russia

Abstract

Complex alleles (CAs) arise when two or more nucleotide variants are present on a single allele. CAs of the CFTR gene complicate the cystic fibrosis diagnosis process, classification of pathogenic variants, and determination of the clinical picture of the disease and increase the need for additional studies to determine their pathogenicity and modulatory effect in response to targeted therapy. For several different populations around the world, characteristic CAs of the CFTR gene have been discovered, although in general the prevalence and pathogenicity of CAs have not been sufficiently studied. This review presents examples of using intestinal organoid models for assessments of the two most common and two rare CFTR CAs in individuals with cystic fibrosis in Russia.

Funder

Federal State Funded Research Institution “Research Centre for Medical Genetics”

Publisher

MDPI AG

Reference47 articles.

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2. (2023, June 19). Cystic Fibrosis Variant Database. Available online: http://www.genet.sickkids.on.ca/.

3. (2023, June 19). Clinical and Functional Translation of CFTR. Available online: https://cftr2.org/mutations_history.

4. Petrova, N.V., Kashirskaya, N.Y., Vasilyeva, T.A., Balinova, N.V., Marakhonov, A.V., Kondratyeva, E.I., Zhekaite, E.K., Voronkova, A.Y., Kutsev, S.I., and Zinchenko, R.A. (2022). High Frequency of Complex CFTR Alleles Associated with c.1521_1523delCTT (F508del) in Russian Cystic Fibrosis Patients. BMC Genom., 23.

5. Krasovsky, S.A., Starinova, M.A., Voronkova, A.Y., Amelina, E.L., Kashirskaya, N.Y., Kondratyeva, E.I., and Nazarenko, L.P. (2023). Register of Patients with Cystic Fibrosis in the Russian Federation. 2021 Year, Publishing House Foundation «Ostrova».

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