Performance in Behavioral Testing in an Animal Model of Post-Surgical Hypoparathyroidism

Author:

Dettori Cristina1,Ronca Francesca1,Di Buono Giulia1,Saba Alessandro1ORCID,Di Lupo Francesca1,Polini Beatrice1ORCID,Ricardi Caterina1,Frascarelli Sabina1,Cetani Filomena2,Marcocci Claudio2,Zucchi Riccardo1,Chiellini Grazia1ORCID,Scalese Marco3ORCID,Saponaro Federica1ORCID

Affiliation:

1. Department of Surgical, Medical and Molecular Pathology and Critical Care, 56126 Pisa, Italy

2. Endocrine Unit, University Hospital of Pisa, 56124 Pisa, Italy

3. Institute of Clinical Physiology, National Council of Research, 56126 Pisa, Italy

Abstract

Background: Hypoparathyroidism (HypoPT) is characterized by hypocalcemia and undetectable/inappropriately low PTH. Post-surgical HypoPT (PS-HypoPT) is the most common cause. Patients with PS-HypoPT present neuropsychological symptoms, probably due to the PTH deprivation in the central nervous system (CNS). However, these mechanisms are still not elucidated. The aim of this study was to evaluate the effects of PTH deprivation on CNS in an animal model of PS-HypoPT via a cognitive/behavioral assessment approach. Methods: A surgical rat model of PS-HypoPT was obtained and treated with calcium to maintain normocalcemia. Twenty PS-HypoPT rats and twenty sham-operated controls (Crl) underwent behavioral testing in a Morris Water Maze (MWM), Open Field (OF), and Elevated Plus Maze (EPM). Results: In the MWM, PTx rats showed a higher Escape Latency Time compared to Crl rats (p < 0.05); we observed a statistically significant improvement in the performance (day 1 to 8 p < 0.001), which was less pronounced in PTx group. In the OF test, the time and distance spent in the zone of interest were significantly lower in the PTx group compared with the Crl (p < 0.01 and p < 0.01). In the EPM experiment, the time spent in the close arm was significantly higher in the PTx group compared with the Crl (p < 0.01). Conclusions: This animal model of PS-HypoPT shows an impairment in spatial memory, which improved after training, and a marked anxiety-like behavior, resembling the condition of patients with PS-HypoPT. Further studies are needed to elucidate mechanisms.

Publisher

MDPI AG

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