Identification of Pathogenic Leptospira kirschneri Serogroup Grippotyphosa in Water Voles (Arvicola terrestris) from Ruminant Pastures in Puy-de-Dôme, Central France
-
Published:2023-02-06
Issue:2
Volume:12
Page:260
-
ISSN:2076-0817
-
Container-title:Pathogens
-
language:en
-
Short-container-title:Pathogens
Author:
Harran Elena12ORCID, Pinot Adrien1, Kodjo Angeli1, Djelouadji Zouheira1, Le Gudayer Marine3, Sionfoungo Daouda Soro1, Groud Karine1, Lattard Virginie1ORCID, Ayral Florence13ORCID
Affiliation:
1. USC 1233-RS2GP, VetAgro Sup, Université de Lyon, 69280 Marcy L’Etoile, France 2. Faculty of Arts and Sciences, Holy Spirit University of Kaslik (USEK), Jounieh P.O. Box 446, Lebanon 3. Laboratoire des Leptospires et d’Analyses Vétérinaires, VetAgro Sup, Université de Lyon, 69280 Marcy L’Etoile, France
Abstract
Rodents are the primary reservoirs for pathogenic Leptospira species, which cause leptospirosis. Among the key potential carriers are water voles, whose population outbreaks can consequently pose a major threat to human and animal health. We studied the prevalence, prominence, and epidemiology of pathogenic Leptospira species in water voles in central France. First, 46 voles were captured, and DNA was extracted from kidney, lung, liver, blood, and urine and tested for the presence of Leptospira using three molecular methods: PCR, O-antigen typing, and variable number tandem repeat (VNTR) typing. We also attempted to culture leptospires from kidney and urine samples. In addition, we investigated leptospiral antibodies in serum samples from 60 sheep using microscopic agglutination testing. These animals co-occurred with the voles, so we sought to assess their degree of exposure and involvement in pathogen dynamics. The overall prevalence of infection was 76.1% (CI95% [61.2%, 87.4%]). The only strain found was L. kirschneri serogroup Grippotyphosa and a similar VNTR profile was acquired. Leptospires were successfully cultured from kidney and urine samples for four voles. Three sheep had low antibody titers against the Leptospira serogroup Grippotyphosa. Taken together, our results suggest the exclusive carriage of L. kirschneri serogroup Grippotyphosa among water voles in central France. Nevertheless, their ability to act as reservoir hosts that transmit the pathogen to co-occurring livestock remains unclear and merits further research.
Subject
Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy
Reference91 articles.
1. Leptospirosis;Levett;Clin. Microbiol. Rev.,2001 2. Costa, F., Hagan, J.E., Calcagno, J., Kane, M., Torgerson, P., Martinez-Silveira, M.S., Stein, C., Abela-Ridder, B., and Ko, A.I. (2015). Global Morbidity and Mortality of Leptospirosis: A Systematic Review. PLoS Negl. Trop. Dis., 9. 3. Leptospirosis: A Zoonotic Disease of Global Importance;Bharti;Lancet Infect. Dis.,2003 4. Leptospirosis Associated with Outbreak of Acute Febrile Illness and Pulmonary Haemorrhage, Nicaragua, 1995;Zaki;Lancet,1996 5. Animal Leptospirosis;Ellis;Curr. Top. Microbiol. Immunol.,2015
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|