Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature-Reference-Cited by-同舟云学术

Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature

Published:2021-09-18 Issue:9 Volume:10 Page:1215
ISSN:2076-0817
Container-title:Pathogens
language:en
Short-container-title:Pathogens

Author:

Markt Rudolf^ORCID,Mayr Markus,Peer Evelyn,Wagner Andreas O.^ORCID,Lackner Nina,Insam Heribert^ORCID

Abstract

SARS-CoV-2 wastewater epidemiology suffers from uncertainties concerning sample storage. We show the effect of the storage of wastewater on the detectable SARS-CoV-2 load. Storage at 4 °C for up to 9 days had no significant effect, while storage at −20 °C led to a significant reduction in gene copy numbers.

Funder

Förderkreis 1669 - Wissenschaft Gesellschaft

Bundesministerium für Bildung, Wissenschaft und Forschung

Publisher

MDPI AG

Subject

Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy

Link

https://www.mdpi.com/2076-0817/10/9/1215/pdf

Reference12 articles.

1. Presence of SARS-Coronavirus-2 RNA in Sewage and Correlation with Reported COVID-19 Prevalence in the Early Stage of the Epidemic in The Netherlands

2. SARS-CoV-2 Titers in Wastewater Are Higher than Expected from Clinically Confirmed Cases