Hypermucoviscous Multidrug-Resistant Klebsiella variicola Strain LL2208 Isolated from Chinese Longsnout Catfish (Leiocassis longirostris): Highly Similar to Human K. variicola Strains

Author:

Li Qingyong1,Yu Xin1,Ye Lin1,Hou Tongyu1,Liu Yi1,Liu Guiming1,Wang Qing23,Zhang Defeng23ORCID

Affiliation:

1. Fisheries Research and Extension Center of Huizhou, Huizhou 516055, China

2. Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China

3. Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China

Abstract

Outbreaks of bacterial diseases occur in farmed Chinese longsnout catfish (Leiocassis longirostris). Due to limited information on aquatic Klebsiella variicola-infected animals, this study aimed to identify strain LL2208 isolated from diseased L. longirostris, determine its biological features, and evaluate its risk to public health. Strain LL2208 was tested for molecular identification, challenge, string, biofilm formation, and antimicrobial susceptibility. Furthermore, the whole genome of the strain was sequenced and analyzed. Based on molecular identification, strain LL2208 was identified as K. variicola. Artificial infection showed that this strain was moderately virulent to L. longirostris with an LD50 = 7.92 × 107 CFU/mL. Antibiotic sensitivity tests showed that this strain was resistant to penicillins, macrolides, aminoglycosides, amphenicols, glycopeptides, and lincosamide, indicating multidrug resistance. Strain LL2208 has a genome size of 5,557,050 bp, with a GC content of 57.38%, harboring 30 antimicrobial resistance genes and numerous virulence-related genes. Its molecular type was ST595-KL16-O5. Collinearity analysis showed that strain LL2208 was highly similar to the human-derived K. variicola strain. In conclusion, the multidrug-resistant and virulent K. variicola strain LL2208 was isolated from fish and may have originated from humans. These results provide a foundation for further studies on the transmission of K. variicola between humans and aquatic animals.

Funder

China Agriculture Research System

Central Public-interest Scientific Institution Basal Research Fund, CAFS

Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams

Publisher

MDPI AG

Reference48 articles.

1. Comprehensive genomic analysis and characterization of a new ST 174 type Klebsiella variicola strain isolated from chicken embryos;Yin;Infect. Genet. Evol.,2021

2. Guo, Y.T., Zhai, Y., Zhang, Z., Li, D.X., Wang, Z.W., Li, J.Q., He, Z.L., Hu, S.N., Kang, Y., and Gao, Z.C. (2018). Complete genomic analysis of a kingdom-crossing Klebsiella variicola isolate. Front. Microbiol., 9.

3. China Antimicrobial Resistance Surveillance System (2023). Antimicrobial resistance surveillance reports from respiratory medicine departments from China Antimicrobial Resistance Surveillance System in 2021. China J. Nosocomiol., 33, 3380–3386. (In Chinese).

4. Isolation, identification, and pathogenic analysis of Klebsiella pneumoniae from large yellow croaker Larimichthys crocea;Pan;Fish. Sci.,2023

5. Isolation and identification of pathogenic Klebsiella pneumoniae from Megalobrama amhlycephala;Teng;J. Hydroecol.,2016

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3