Persistence of Tembusu Virus in Culex tritaeniorhynchus in Yunnan Province, China-Reference-Cited by-同舟云学术

Persistence of Tembusu Virus in Culex tritaeniorhynchus in Yunnan Province, China

Published:2023-03-21 Issue:3 Volume:12 Page:490
ISSN:2076-0817
Container-title:Pathogens
language:en
Short-container-title:Pathogens

Author:

Hu Danhe¹²,Wu Chao³,Wang Ruichen²,Yao Xiaohui¹²,Nie Kai²^ORCID,Lv Quan³,Fu Shihong²,Yin Qikai²^ORCID,Su Wenzhe⁴^ORCID,Li Fan²,Xu Songtao²,He Ying²,Liang Guodong²,Li Xiangdong¹⁵^ORCID,Wang Huanyu²^ORCID

Affiliation:

1. Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China

2. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

3. Yunnan Institute of Parasitic Diseases, Pu’er 665000, China

4. Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China

5. Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin, College of Life Sciences, Tarim University, Alar 843399, China

Abstract

The Tembusu virus (TMUV), a member of the Flaviviridae family, can be transmitted via mosquitoes and cause poultry disease. In 2020, a strain of TMUV (YN2020-20) was isolated from mosquito samples collected in Yunnan province, China. In vitro experiments showed that TMUV-YN2020-20 produced a significant cytopathic effect (CPE) in BHK, DF-1, and VERO cells, while the CPE in C6/36 cells was not significant. Phylogenetic analysis revealed that the strain belonged to Cluster 3.2 and was closely related to the Yunnan mosquito-derived isolates obtained in 2012 and the Shandong avian-derived isolate obtained in 2014. Notably, TMUV-YN2020-20 developed five novel mutations (E-V358I, NS1-Y/F/I113L, NS4A-T/A89V, NS4B-D/E/N/C22S, and NS5-E638G) at loci that were relatively conserved previously. The results of this study demonstrate the continuous circulation and unique evolution of TMUV in mosquitoes in Yunnan province and suggest that appropriate surveillance should be taken.

Funder

National Key Research and Development Program of China

Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions

Development Grant of State Key Laboratory of Infectious Disease Prevention and Control

United States National Institutes of Health U01

Publisher

MDPI AG

Subject

Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy

Link

https://www.mdpi.com/2076-0817/12/3/490/pdf

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