Prevalence and Characterization of Salmonella Isolated from Chickens in Anhui, China
-
Published:2023-03-16
Issue:3
Volume:12
Page:465
-
ISSN:2076-0817
-
Container-title:Pathogens
-
language:en
-
Short-container-title:Pathogens
Author:
Shen Xuehuai12ORCID, Yin Lei2, Zhang Anyun3ORCID, Zhao Ruihong2, Yin Dongdong2, Wang Jieru2ORCID, Dai Yin2, Hou Hongyan2, Pan Xiaocheng2, Hu Xiaomiao2, Zhang Danjun2, Liu Yongjie1
Affiliation:
1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China 2. Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230001, China 3. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610017, China
Abstract
Salmonella is one of the most important zoonotic pathogens that can cause both acute and chronic illnesses in poultry flocks, and can also be transmitted to humans from infected poultry. The purpose of this study was to investigate the prevalence, antimicrobial resistance, and molecular characteristics of Salmonella isolated from diseased and clinically healthy chickens in Anhui, China. In total, 108 Salmonella isolates (5.66%) were successfully recovered from chicken samples (n = 1908), including pathological tissue (57/408, 13.97%) and cloacal swabs (51/1500, 3.40%), and S. Enteritidis (43.52%), S. Typhimurium (23.15%), and S. Pullorum (10.19%) were the three most prevalent isolates. Salmonella isolates showed high rates of resistance to penicillin (61.11%), tetracyclines (47.22% to tetracycline and 45.37% to doxycycline), and sulfonamides (48.89%), and all isolates were susceptible to imipenem and polymyxin B. In total, 43.52% isolates were multidrug-resistant and had complex antimicrobial resistance patterns. The majority of isolates harbored cat1 (77.78%), blaTEM (61.11%), and blaCMY-2 (63.89%) genes, and the antimicrobial resistance genes in the isolates were significantly positively correlated with their corresponding resistance phenotype. Salmonella isolates carry high rates of virulence genes, with some of these reaching 100% (invA, mgtC, and stn). Fifty-seven isolates (52.78%) were biofilm-producing. The 108 isolates were classified into 12 sequence types (STs), whereby ST11 (43.51%) was the most prevalent, followed by ST19 (20.37%) and ST92 (13.89%). In conclusion, Salmonella infection in chicken flocks is still serious in Anhui Province, and not only causes disease in chickens but might also pose a threat to public health security.
Funder
Key Research and Development Project of Anhui Province Major Science and Technology Special Project in Anhui Province The China Agriculture Research System of MOF and MARA Anhui Poultry Industry Technical System Project Platform Project of Anhui Academy of Agricultural Science
Subject
Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy
Reference86 articles.
1. Humoral immunity vs. Salmonella;Takaya;Front. Immunol.,2019 2. Alikhan, N.F., Moreno, L.Z., Castellanos, L.R., Chattaway, M.A., McLauchlin, J., Lodge, M., O’Grady, J., Zamudio, R., Doughty, E., and Petrovska, L. (2022). Dynamics of Salmonella enterica and antimicrobial resistance in the Brazilian poultry industry and global impacts on public health. PLoS Genet., 18. 3. Argüello, H., Guerra, B., Rodríguez, I., Rubio, P., and Carvajal, A. (2018). Characterization of antimicrobial resistance determinants and class 1 and class 2 integrons in Salmonella enterica spp., multidrug-resistant isolates from pigs. Genes, 9. 4. Food recalls associated with foodborne disease outbreaks, United States, 2006–2016;Qiu;Epidemiol. Infect.,2021 5. Salmonellosis: The role of poultry meat;Antunes;Clin. Microbiol. Infect.,2016
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|