Abstract
The hepatitis B virus (HBV), belonging to the Hepadnaviridae family, is responsible for a global health concern still in the 21st century. The virus is divided into 10 genotypes, which differ in geographical distribution and in their effect on disease progression and transmission, susceptibility to mutations, and response to treatment. There are many methods for diagnostics of HBV and differentiating its genotypes. Various commercial kits based on real-time polymerase chain reaction (RT PCR) and hybridization available, as well as whole genome sequencing or the sequencing of only individual parts of the genomes. We compared a commercial kit AmpliSens HBV-genotype-FRT, based on RT PCR, with an adapted method of amplification of the surface genomic region combined with Sanger sequencing. In the examined samples we identified the A, B, C, D, and E genotypes. By PCR with Sanger sequencing, the genotypes were determined in all 103 samples, while by using the commercial kit we successfully genotyped only 95 samples, including combined genotypes, which we could not detect by sequencing.
Funder
Ministry of Education, Science, Research and Sport of the Slovak Republic
Subject
Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy
Reference45 articles.
1. Hepatitis B and C in the EU Neighbourhood: Prevalence, Burden of Disease and Screening Policieshttps://www.ecdc.europa.eu/en/publications-data/hepatitis-b-and-c-eu-neighbourhood-prevalence-burden-disease-and-screening
2. Prevalence and Risk Factors for Hepatitis B Virus Infection in Roma and Non-Roma People in Slovakia
3. High Hepatitis B and Low Hepatitis C Prevalence in Roma Population in Eastern Slovakia
4. Hepatitis B—Annual Epidemiological Report 2019https://www.ecdc.europa.eu/en/publications-data/hepatitis-b-annual-epidemiological-report-2019
5. Annual Report of Regional Public Health Authorities in Slovakia for the Year 2020,2021
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献