Prion Seeding Activity in Plant Tissues Detected by RT-QuIC-Reference-Cited by-同舟云学术

Prion Seeding Activity in Plant Tissues Detected by RT-QuIC

Published:2024-05-26 Issue:6 Volume:13 Page:452
ISSN:2076-0817
Container-title:Pathogens
language:en
Short-container-title:Pathogens

Author:

Burgener Kate¹²^ORCID,Lichtenberg Stuart Siegfried³⁴^ORCID,Walsh Daniel P.⁵^ORCID,Inzalaco Heather N.⁶,Lomax Aaron⁷⁸,Pedersen Joel A.¹²⁷

Affiliation:

1. Molecular and Environmental Toxicology Program, University of Wisconsin-Madison, Madison, WI 53706, USA

2. Department of Environmental Health and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA

3. Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN 55108, USA

4. Minnesota Center for Prion Research and Outreach, University of Minnesota, St. Paul, MN 55108, USA

5. U.S. Geological Survey, Montana Cooperative Wildlife Research Unit, University of Montana, Missoula, MT 59812, USA

6. Wisconsin Cooperative Wildlife Research Unit, Department of Forest and Wildlife Ecology, University of Wisconsin-Madison, Madison, WI 53706, USA

7. Department of Soil Science, University of Wisconsin-Madison, Madison, WI 53706, USA

8. Varizymes, Middleton, WI 53562, USA

Abstract

Prion diseases such as scrapie, bovine spongiform encephalopathy (BSE), and chronic wasting disease (CWD) affect domesticated and wild herbivorous mammals. Animals afflicted with CWD, the transmissible spongiform encephalopathy of cervids (deer, elk, and moose), shed prions into the environment, where they may persist and remain infectious for years. These environmental prions may remain in soil, be transported in surface waters, or assimilated into plants. Environmental sampling is an emerging area of TSE research and can provide more information about prion fate and transport once shed by infected animals. In this study, we have developed the first published method for the extraction and detection of prions in plant tissue using the real-time quaking-induced conversion (RT-QuIC) assay. Incubation with a zwitterionic surfactant followed by precipitation with sodium phosphotungstate concentrates the prions within samples and allows for sensitive detection of prion seeding activity. Using this protocol, we demonstrate that prions can be detected within plant tissues and on plant surfaces using the RT-QuIC assay.

Funder

Chronic Wasting Disease Alliance, a project of the Wildlife Management Institute

Publisher

MDPI AG

Link

https://www.mdpi.com/2076-0817/13/6/452/pdf

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