Comparison of Three Serologic Tests for the Detection of Anti-Coxiella burnetii Antibodies in Patients with Q Fever-Reference-Cited by-同舟云学术

Comparison of Three Serologic Tests for the Detection of Anti-Coxiella burnetii Antibodies in Patients with Q Fever

Published:2023-06-26 Issue:7 Volume:12 Page:873
ISSN:2076-0817
Container-title:Pathogens
language:en
Short-container-title:Pathogens

Author:

França Danilo Alves de¹^ORCID,Mioni Mateus de Souza Ribeiro¹^ORCID,Fornazari Felipe¹,Rodrigues Nássarah Jabur Lot¹,Polido Lucas Roberto Ferreira²,Appolinario Camila Michele¹,Ribeiro Bruna Letícia Devidé¹,Duré Ana Íris de Lima³^ORCID,Silva Marcos Vinicius Ferreira³,Richini-Pereira Virgínia Bodelão⁴^ORCID,Langoni Helio¹^ORCID,Megid Jane¹

Affiliation:

1. Department of Veterinary Hygiene and Public Health, São Paulo State University, Botucatu 05508-220, Brazil

2. Botucatu Medical School, São Paulo State University, Botucatu 05508-220, Brazil

3. Ezequiel Dias Foundation, Otávio Magalhães Institute, Belo Horizonte 30510-010, Brazil

4. Regional Laboratories Center II, Adolfo Lutz Institute, Bauru 17015-110, Brazil

Abstract

The performance of a commercial immunofluorescence assay (IFA commercial), an in-house immunofluorescence assay (IFA in-house) and an indirect enzyme-linked immunosorbent assay (ELISA) were evaluated in the detection of antibodies anti-C. burnetii in the serum of Q fever patients and persons without the disease. For the study, seropositive and seronegative samples for Q fever (n = 200) from a serum bank of the Instituto Adolfo Lutz in Brazil were used. Commercial IFA was considered in this study as the gold standard for diagnosing Q fever. The in-house IFA demonstrated good agreement with the commercial test, showing high sensitivity (91%) and specificity (97%) compared to the gold standard, with a Kappa coefficient of 0.8954. The indirect ELISA test showed lower agreement with the gold standard, showing low sensitivity (67%), although the specificity of the technique was high (97%) and the Kappa coefficient was moderate (0.6631). In-house IFA is an excellent alternative for diagnosing Q fever.

Funder

National Council for Scientific and Technological Development

Publisher

MDPI AG

Subject

Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy

Link

https://www.mdpi.com/2076-0817/12/7/873/pdf

Reference21 articles.

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2. Beare, P.A., Jeffrey, B.M., Long, C.M., Martens, C.M., and Heinzen, R.A. (2018). Genetic mechanisms of Coxiella burnetii lipopolysaccharide phase variation. PLoS Pathog., 14.

3. França, D.A., Mioni, M.S.R., Fornazari, F., Duré, A.Í.L., Silva, M.V.F., Possebon, F.S., Richini-Pereira, V.B., Langoni, H., and Megid, J. (2022). Seropositivity for Coxiella burnetii in suspected patients with dengue in São Paulo state, Brazil. PLoS Negl. Trop Dis., 16.