Study of the Genetic Expression of Antiretroviral Restriction Factors and Acute Phase Proteins in Cattle Infected with Bovine Leukemia Virus

Author:

González-Méndez Ana S.1ORCID,Tórtora Pérez Jorge L.1,Rojas-Anaya Edith2ORCID,Ramírez Álvarez Hugo1ORCID

Affiliation:

1. Virology, Genetics and Molecular Biology Laboratory, Faculty of Higher Education Cuautitlan, Veterinary Medical School, Campus 4, National Autonomous University of Mexico, Cuautitlan Izcalli, Mexico City CP 54714, Mexico

2. Pacific Center Research Center, INIFAP, Guadalajara CP 44660, Mexico

Abstract

The goal of this study was to analyze the genetic expression of antiretroviral restriction factors (ARF) and acute phase proteins (APP), as well as their correlation with proviral and viral loads in cattle with aleukemic (AL) and persistent lymphocytosis (PL). Complete blood samples were collected from a herd of dairy cows, and we extracted genetic material from peripheral blood leukocytes. Absolute quantification of the expression of ARF (APOBEC-Z1, Z2, and Z3; HEXIM-1, HEXIM-2, and BST2) and APP (haptoglobin (HP), and serum amyloid A (SAA)) was performed by qPCR. Statistical significance was observed in the expression of APOBEC-Z3 in BLV-infected animals. We only found positive correlations with a strong expression of the ARF genes in the AL group. The participation of APOBEC (Z1 and Z3), HEXIM-1, and HEXIM-2 was more frequently identified in BLV-infected animals. HEXIM-2 showed active gene expression in the AL group. Although the expression of ARF in early stages of infection (AL) maintains an important participation, in late stages (PL) it seems to have little relevance.

Funder

UNAM-DGAPA-PAPIIT

FESC. UNAM

Publisher

MDPI AG

Subject

Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy

Reference56 articles.

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