Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico-Reference-Cited by-同舟云学术

Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico

Published:2023-06-16 Issue:6 Volume:12 Page:835
ISSN:2076-0817
Container-title:Pathogens
language:en
Short-container-title:Pathogens

Author:

Dehesa-Canseco Freddy¹,Pastrana-Unzueta Roxana¹,Carrillo-Guzmán Nadia¹,Liljehult-Fuentes Francisco¹,Pérez-De la Rosa Juan Diego²,Ramírez-Mendoza Humberto³,Estrada-Franco Jose Guillermo⁴,Navarro-López Roberto¹,Hernández Jesús⁵^ORCID,Solís-Hernández Mario¹

Affiliation:

1. Comisión México-Estados Unidos para la Prevención de la Fiebre Aftosa y otras Enfermedades Exóticas de los Animales (CPA), Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA), Secretaría de Agricultura y Desarrollo Rural (SADER), Ciudad de Mexico 05110, Mexico

2. Centro Nacional de Servicios de Constatación en Salud Animal (CENAPA), Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA), Secretaría de Agricultura y Desarrollo Rural (SADER), Cuernavaca 62574, Mexico

3. Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Ciudad de Mexico 04510, Mexico

4. Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa 88710, Mexico

5. Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A. C. (CIAD), Hermosillo Sonora 83304, Mexico

Abstract

SARS-CoV-2 mainly affects humans; however, it is important to monitor the infection of companion and wild animals as possible reservoirs of this virus. In this sense, seroprevalence studies in companion animals, such as dogs and cats, provide important information about the epidemiology of SARS-CoV-2. This study aimed to evaluate the seroprevalence of neutralizing antibodies (nAbs) against the ancestral strain and the Omicron BA.1 subvariant in dogs and cats in Mexico. Six hundred and two samples were obtained from dogs (n = 574) and cats (n = 28). These samples were collected from the end of 2020 to December 2021 from different regions of Mexico. The presence of nAbs was evaluated using a plaque reduction neutralization test (PRNT) and microneutralization (MN) assays. The results showed that 14.2% of cats and 1.5% of dogs presented nAbs against the ancestral strain of SARS-CoV-2. The analysis of nAbs against Omicron BA.1 in cats showed the same percentage of positive animals but a reduced titer. In dogs, 1.2% showed nAbs against Omicron BA.1. These results indicate that nAbs were more frequent in cats than in dogs and that these nAbs have a lower capacity to neutralize the subvariant Omicron BA.1.

Funder

Servicio Nacional de Sanidad, Inocuidad, and Calidad Agroalimentaria

SIP-IPN

Publisher

MDPI AG

Subject

Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy

Link

https://www.mdpi.com/2076-0817/12/6/835/pdf

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