Resistance Profiles and Virulence Determinants in Biofilm-Forming Enterococcus faecium Isolated from Raw Seafood in Bangladesh

Author:

Ullah Md. Ashek1ORCID,Islam Md. Saiful1ORCID,Rana Md. Liton1ORCID,Ferdous Farhana Binte1ORCID,Neloy Fahim Haque1,Firdous Zannatul1,Hassan Jayedul1ORCID,Rahman Md. Tanvir1ORCID

Affiliation:

1. Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Abstract

Pathogenic, antibiotic-resistant, and biofilm-forming bacteria can be transferred to humans through the consumption of contaminated seafood. The present study was carried out to determine antibiotic resistance profiles and virulence determinants in biofilm-forming Enterococcus faecium isolated from seafood in Bangladesh. A total of 150 seafood samples, including shrimp (n = 50), crabs (n = 25), and marine fish (n = 75), were screened using cultural, staining, biochemical, polymerase chain reaction (PCR), Congo red (CR), and disk diffusion (DD) assays. In PCR, E. faecium was detected in 27.3% (41/150; CI95% 20.8; 34.9) of samples, where marine fish (34.7%, CI95% 24.9; 45.9) had the highest prevalence (p < 0.05) compared to crabs (32%, CI95% 17.2; 51.6) and shrimp (14%, CI95% 7.0; 26.1). Thirty-two (78.1%, CI95% 63.3; 88.0) of the E. faecium isolates were determined to be biofilm formers in the CR test, where 43.9% (18/41, CI95% 29.9; 59.0) and 34.2% (14/41, CI95% 21.6; 49.5) of the isolates were strong and intermediate biofilm formers, respectively. In PCR, virulence genes, i.e., pil (100%), ace (92.7%), agg (68.3%), fsrA (65.9%), gelE (63.4%), sprE (53.7%), fsrB (51.2%), and fsrC (43.9%), were detected in E. faecium isolates. All the E. faecium isolates were phenotypically resistant to ≥3 antimicrobial categories and ≥3 antibiotics, including WHO-classified reserve antibiotics linezolid (70.7%) and fosfomycin (19.5%). Moreover, the multiple antibiotic resistance index ranged up to 0.8, showing resistance to ten antibiotics and eight antibiotic classes. In this study, the prevalence of virulence genes and antibiotic resistance was significantly greater (p < 0.05) in strong biofilm-forming E. faecium strains as compared to strains with intermediate and non-biofilm-forming abilities. As far as we know, this study, for the first time in Bangladesh, determined antibiotic resistance and detected virulence genes in biofilm-forming E. faecium isolated from seafood samples. The data from this study could play a significant role in evaluating potential health hazards linked to the ingestion of uncooked or minimally processed seafood.

Funder

Bangladesh Agricultural University Research System

Publisher

MDPI AG

Subject

Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy

Reference53 articles.

1. Exploitation and conservation of coastal and marine fisheries in Bangladesh: Do the fishery laws matter?;Islam;Mar. Policy,2017

2. Department of Fisheries (DoF) (2023, August 02). Yearbook of Fisheries Statistics of Bangladesh, 2021–2022, Fisheries Resources Survey System (FRSS), 2023, Volume 39, Ministry of Fisheries, Bangladesh, Available online: http://fisheries.portal.gov.bd/site/download/42836060-aa5e-491d-8309-cf750886813b.

3. Major food borne pathogens in fish and fish products: A review;Novoslavskij;Ann. Microbiol.,2016

4. Antimicrobial resistance, virulence determinants, and biofilm formation of Enterococcus species from ready-to-eat seafood;Igbinosa;Front. Microbiol.,2019

5. Diversity of species and antibiotic resistance in enterococci isolated from seafood in Tunisia;Hamdaoui;Ann. Microbiol.,2017

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