Impact of Sulfated Hyaluronan on Bone Metabolism in Diabetic Charcot Neuroarthropathy and Degenerative Arthritis
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Published:2022-12-02
Issue:23
Volume:23
Page:15146
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Schulze Sabine, Neuber ChristinORCID, Möller Stephanie, Hempel Ute, Hofbauer Lorenz C., Schaser Klaus-Dieter, Pietzsch JensORCID, Rammelt StefanORCID
Abstract
Bone in diabetes mellitus is characterized by an altered microarchitecture caused by abnormal metabolism of bone cells. Together with diabetic neuropathy, this is associated with serious complications including impaired bone healing culminating in complicated fractures and dislocations, especially in the lower extremities, so-called Charcot neuroarthropathy (CN). The underlying mechanisms are not yet fully understood, and treatment of CN is challenging. Several in vitro and in vivo investigations have suggested positive effects on bone regeneration by modifying biomaterials with sulfated glycosaminoglycans (sGAG). Recent findings described a beneficial effect of sGAG for bone healing in diabetic animal models compared to healthy animals. We therefore aimed at studying the effects of low- and high-sulfated hyaluronan derivatives on osteoclast markers as well as gene expression patterns of osteoclasts and osteoblasts from patients with diabetic CN compared to non-diabetic patients with arthritis at the foot and ankle. Exposure to sulfated hyaluronan (sHA) derivatives reduced the exaggerated calcium phosphate resorption as well as the expression of genes associated with bone resorption in both groups, but more pronounced in patients with CN. Moreover, sHA derivatives reduced the release of pro-inflammatory cytokines in osteoclasts of patients with CN. The effects of sHA on osteoblasts differed only marginally between patients with CN and non-diabetic patients with arthritis. These results suggest balancing effects of sHA on osteoclastic bone resorption parameters in diabetes.
Funder
Deutsche Forschungsgemeinschaft
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference41 articles.
1. An overview of Charcot’s neuroarthropathy;Dardari;J. Clin. Transl. Endocrinol.,2020 2. Ahluwalia, R., Bilal, A., Petrova, N., Boddhu, K., Manu, C., Vas, P., Bates, M., Corcoran, B., Reichert, I., and Mulholland, N. (2020). The Role of Bone Scintigraphy with SPECT/CT in the Characterization and Early Diagnosis of Stage 0 Charcot Neuroarthropathy. J. Clin. Med., 9. 3. Rammelt, S. (2022, July 13). Management of Acute Hindfoot Fractures in Diabetics. Available online: https://link.springer.com/chapter/10.1007/978-3-319-27623-6_7. 4. Five-year mortality in patients with diabetic foot ulcer during 2009–2010 was lower than expected;Amadou;Diabetes Metab.,2020 5. The role of proinflammatory cytokines in the cause of neuropathic osteoarthropathy (acute Charcot foot) in diabetes;Jeffcoate;Lancet,2005
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