Prospective Evaluation of the BD MAX StaphSR Assay for the Screening of Methicillin-Susceptible and -Resistant Staphylococcus aureus from Nasal Swabs Taken in Intensive Care Unit Patients

Author:

Fayolle Martin123ORCID,Epercieux Amélie2,Haddar Cyrille H.2,Pillet Sylvie123ORCID,Berthelot Philippe124ORCID,Pozzetto Bruno123ORCID,Carricajo Anne123,Grattard Florence123,Verhoeven Paul O.123ORCID

Affiliation:

1. CIRI, Centre International de Recherche en Infectiologie, GIMAP Team, Inserm, U1111, CNRS, UMR5308, ENS Lyon, Claude Bernard Lyon 1 University, 69007 Lyon, France

2. Faculty of Medicine, Jean Monnet St-Etienne University, 42023 St-Etienne, France

3. Department of Infectious Agents and Hygiene, University Hospital of St-Etienne, 42055 St-Etienne, France

4. Infection Control Unit, Department of Infectious Diseases, University Hospital of St-Etienne, 42055 St-Etienne, France

Abstract

Screening patients for S. aureus nasal carriage has proved effective in preventing cross-contamination and endogenous infection with this bacterium. The aim of this study was to assess the performance of the BD MAX StaphSR assay with liquid Amies elution swabs, taken during routine care of intensive care unit patients. Direct and pre-enriched cultures were used as reference methods to screen for S. aureus and methicillin-resistant S. aureus (MRSA). Discrepant results between the BD MAX StaphSR assay and cultures were resolved by using the Xpert SA Nasal Complete assay. A total of 607 nasal swabs taken from 409 patients were included in this study. Compared to culture methods, the sensitivity and specificity of the BD MAX StaphSR assay were 92.5% and 91.7% for S. aureus screening, and 94.7% and 98.3% for MRSA screening, respectively. In 52 (8.6%) specimens, there was a discrepancy between the results of cultures and the BD MAX StaphSR assay, including 13 (25%) where the results of the BD MAX StaphSR assay were confirmed by the Xpert SA Nasal Complete test. This prospective study showed that the BD MAX StaphSR assay is reliable for S. aureus and MRSA detection from nasal samples taken with liquid Amies elution swabs.

Funder

Becton Dickinson

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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