Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis-Reference-Cited by-同舟云学术

Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis

Published:2023-09-21 Issue:18 Volume:24 Page:14363
ISSN:1422-0067
Container-title:International Journal of Molecular Sciences
language:en
Short-container-title:IJMS

Author:

Wróbel Monika¹^ORCID,Zuzanna Zielińska²,Ołdak Łukasz²³^ORCID,Kalicka Aleksandra⁴^ORCID,Mańka Grzegorz⁵,Kiecka Mariusz⁵,Spaczyński Robert Z.⁶^ORCID,Piekarski Piotr⁷,Banaszewska Beata⁸,Jakimiuk Artur⁹¹⁰^ORCID,Issat Tadeusz¹¹^ORCID,Rokita Wojciech¹²¹³,Młodawski Jakub¹²¹³^ORCID,Szubert Maria¹⁴¹⁵,Sieroszewski Piotr¹⁴¹⁶,Raba Grzegorz¹⁷¹⁸^ORCID,Szczupak Kamil¹⁷¹⁸,Kluz Tomasz¹⁹,Kluza Marek¹⁹,Pierzyński Piotr²⁰,Wojtyła Cezary²⁰^ORCID,Lipa Michał²¹,Warzecha Damian²⁰²¹^ORCID,Wielgoś Mirosław²²²³^ORCID,Sawicki Włodzimierz²⁴,Gorodkiewicz Ewa³,Laudański Piotr²⁰²⁴²⁵

Affiliation:

1. 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, 02-015 Warsaw, Poland

2. Bioanalysis Laboratory, Doctoral School of Exact and Natural Science, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, Poland

3. Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, Poland

4. Faculty of Medicine, Medical University of Warsaw, Żwirki i Wigury 61, 02-091 Warsaw, Poland

5. Angelius Provita Hospital, 40-611 Katowice, Poland

6. Center for Gynecology, Obstetrics and Infertility Treatment Pastelova, 60-198 Poznan, Poland

7. Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 61-701 Poznan, Poland

8. Chair and Department of Laboratory Diagnostics, Poznan University of Medical Sciences, 61-701 Poznan, Poland

9. Department of Reproductive Health, Institute of Mother and Child in Warsaw, 01-211 Warsaw, Poland

10. Department of Obstetrics and Gynecology, Central Clinical Hospital of the Ministry of Interior, 02-507 Warsaw, Poland

11. Department of Obstetrics and Gynecology, Institute of Mother and Child in Warsaw, 01-211 Warsaw, Poland

12. Collegium Medicum, Jan Kochanowski University in Kielce, 25-516 Kielce, Poland

13. Clinic of Obstetrics and Gynecology, Provincial Combined Hospital in Kielce, 25-736 Kielce, Poland

14. Department of Gynecology and Obstetrics, Medical University of Lodz, 90-419 Lodz, Poland

15. Department of Surgical Gynecology and Oncology, Medical University of Lodz, 90-419 Lodz, Poland

16. Department of Fetal Medicine and Gynecology, Medical University of Lodz, 90-419 Lodz, Poland

17. Clinic of Obstetric and Gynecology in Przemysl, 37-700 Przemysl, Poland

18. Department of Obstetrics and Gynecology, University of Rzeszow, 35-310 Rzeszow, Poland

19. Department of Gynecology, Gynecology Oncology and Obstetrics, Institute of Medical Sciences, Medical College of Rzeszow University, 35-310 Rzeszow, Poland

20. OVIklinika Infertility Center, 31 Połczyńska Street, 01-377 Warsaw, Poland

21. City South Hospital Warsaw, 02-781 Warsaw, Poland

22. Premium Medical Clinic, 04-359 Warsaw, Poland

23. Medical Faculty, Lazarski University, 02-662 Warsaw, Poland

24. Department of Obstetrics, Gynecology and Gynecological Oncology, Medical University of Warsaw, 03-242 Warsaw, Poland

25. Women’s Health Research Institute, Calisia University, 62-800 Kalisz, Poland

Abstract

Endometriosis is a chronic disease in which the endometrium cells are located outside the uterine cavity. The aim of this study was to evaluate circulating 20S proteasome and 20S immunoproteasome levels in plasma and peritoneal fluid in women with and without endometriosis in order to assess their usefulness as biomarkers of disease. Concentrations were measured using surface plasmon resonance imaging biosensors. Patients with suspected endometriosis were included in the study—plasma was collected in 112 cases and peritoneal fluid in 75. Based on the presence of endometriosis lesions detected during laparoscopy, patients were divided into a study group (confirmed endometriosis) and a control group (patients without endometriosis). Proteasome and immunoproteasome levels in both the plasma (p = 0.174; p = 0.696, respectively) and the peritoneal fluid (p = 0.909; p = 0.284, respectively) did not differ between those groups. There was a statistically significant difference in the plasma proteasome levels between patients in the control group and those with mild (Stage I and II) endometriosis (p = 0.047) and in the plasma immunoproteasome levels in patients with ovarian cysts compared to those without (p = 0.017). The results of our study do not support the relevance of proteasome and immunoproteasome determination as biomarkers of the disease but suggest a potentially active role in the pathogenesis of endometriosis.

Funder

Polish Ministry of Health

European Union’s Horizon 2020 Research and Innovation Program

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Link

https://www.mdpi.com/1422-0067/24/18/14363/pdf

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