Comparative Studying of Leaf Trichomes, Teeth and Glands in Populus nigra L., Populus deltoides W. Bartram ex Marshall and Their Hybrids

Abstract

Poplars from Aigeiros Duby section are very widespread in the world. A range of morphological characters were studied in such species of this section as Populus deltoides Bartram ex Marshall, P. nigra L. and their hybrid P. × canadensis Moench. However, there is little information about micromorphological characters of their leaves. The aim of this work was to study these characters and understand their species-specific potential. Thus, the morphological features, density and distribution of non-glandular trichoms, marginal glandular trichomes (salicoid teeth or coleters), epiglandular trichomes and basilaminar nectaries-glands were ontogenetically examined by both light and scanning electron microscopy in the certified by molecular markers P. deltoides, P. nigra and P. × canadensis samples. Non-glandular trichomes belong to the uni-, multicellular, uniseriate category. Marginal glandular trichomes can be classified as coleter types. Other morphological and anatomical trichome features are discussed with regard to their possible function. In summary, some variations in leaf morphology may be useful for the P. nigra, P. deltoides and their hybrid P. × canadensis species identification. These species differ in shape and number of basilaminar glands, as well as non-glandular trichome types and their distribution on the leaf.