Analysis of Morphological, Physiological, and Biochemical Traits of Salt Stress Tolerance in Asian Rice Cultivars at Seedling and Early Vegetative Stages

Abstract

Rice (Oryza sativa L.) is a primary energy food for the Asian population. One of the greatest constraints in rice production is soil salinity because rice is very susceptible to salt. Meanwhile, many agricultural lands in Asia are in saline areas. It is important to identify and develop salt-tolerant rice varieties that highly adapt to Asian climates. By combining morphological, physiological, and biochemical assessments for screening the salt tolerance of 116 Asian rice cultivars, we were able to classify them into tolerant, moderate, and sensitive rice cultivars under salinity stress conditions and also understand salt tolerance mechanisms. The rice cultivars that are salt-tolerant include Pokkali from India, TCCP 266 and IR 45427 from the Philippines, and Namyang 7 from Republic of Korea. However, salt-sensitive rice varieties like IR29 and IR58 are from the Philippines, and Daegudo and Guweoldo are from Korea. The salt-tolerant varieties showed signs of tolerance, including a lower percent reduction in germination percentage, root length, root fresh weight, shoot length, plant biomass, and chlorophyll content. In order to maintain the cellular osmotic balance under saline conditions, the salt-tolerant varieties exhibited less membrane damage, a lower Na/K ratio, high proline and sugar accumulation, and lower levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2). Pokkali from India, TCCP 266 and IR 45427 from the Philippines, and Namyang 7 from Republic of Korea are recommended as valuable germplasm resources for Asian rice breeding programs in saline agricultural areas.