L-Lactate Electrochemical Biosensor Based on an Integrated Supramolecular Architecture of Multiwalled Carbon Nanotubes Functionalized with Avidin and a Recombinant Biotinylated Lactate Oxidase

Author:

Tamborelli Alejandro12,Mujica Michael López2,Amaranto Marilla3ORCID,Barra José Luis3,Rivas Gustavo2,Godino Agustina3,Dalmasso Pablo1

Affiliation:

1. CIQA, CONICET, Departamento de Ingeniería Química, Facultad Regional Córdoba, Universidad Tecnológica Nacional, Maestro López esq. Cruz Roja Argentina, Córdoba 5016, Argentina

2. INFIQC, CONICET-UNC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba 5000, Argentina

3. CIQUIBIC, CONICET-UNC, Departamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba 5000, Argentina

Abstract

L-Lactate is an important bioanalyte in the food industry, biotechnology, and human healthcare. In this work, we report the development of a new L-lactate electrochemical biosensor based on the use of multiwalled carbon nanotubes non-covalently functionalized with avidin (MWCNT-Av) deposited at glassy carbon electrodes (GCEs) as anchoring sites for the bioaffinity-based immobilization of a new recombinant biotinylated lactate oxidase (bLOx) produced in Escherichia coli through in vivo biotinylation. The specific binding of MWCNT-Av to bLOx was characterized by amperometry, surface plasmon resonance (SPR), and electrochemical impedance spectroscopy (EIS). The amperometric detection of L-lactate was performed at −0.100 V, with a linear range between 100 and 700 µM, a detection limit of 33 µM, and a quantification limit of 100 µM. The proposed biosensor (GCE/MWCNT-Av/bLOx) showed a reproducibility of 6.0% and it was successfully used for determining L-lactate in food and enriched serum samples.

Funder

ANPCyT-FONCyT

CONICET

MinCyT Córdoba-UNC

SECyT-UNC

SCTyP-UTN

Publisher

MDPI AG

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