Extraction-Free RT-PCR Surveillance Testing and Reporting for SARS-CoV-2-Reference-Cited by-同舟云学术

Extraction-Free RT-PCR Surveillance Testing and Reporting for SARS-CoV-2

Published:2023-07-21 Issue:7 Volume:3 Page:1031-1041
ISSN:2673-8112
Container-title:COVID
language:en
Short-container-title:COVID

Author:

Carney Patrick R.¹²^ORCID,Duellman Tyler³,Chan Jia-Yi³,Wells Lauren³,Tessmer Michael⁴,Frater-Rubsam Leah³^ORCID,Zeller Molly³^ORCID,Field Mark⁴,Speers James³^ORCID,Tyrrell Kelly⁵,Thompson Luke⁶,Bondurant Michael⁴,Morin Tami⁶,Dagnon Tamra⁶,Goff Brian⁶,Runde Corissa⁴,Splinter-Bondurant Sandra³,Konsitzke Charles³,Kelly Patrick⁶,Bradfield Christopher A.¹²³,Hyman Joshua³

Affiliation:

1. Molecular and Environmental Toxicology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, USA

2. Department of Oncology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, USA

3. Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, USA

4. Division of Information Technology, University of Wisconsin-Madison, Madison, WI 53706, USA

5. University Communications, University of Wisconsin-Madison, Madison, WI 53706, USA

6. University Health Services, University of Wisconsin-Madison, Madison, WI 53706, USA

Abstract

The COVID-19 pandemic necessitated sensitive, fast, and inexpensive testing for the virus in 2020 prior to the widespread availability of vaccines. Early testing efforts were limited by bottlenecks on reagents, low-throughput testing options, and the slow return of test results. In this paper, we detail the testing pipeline we established at the University of Wisconsin-Madison for rapid, inexpensive, and sensitive surveillance testing for SARS-CoV-2, and we highlight the strengths of the platform that would allow it to be applied to other disease surveillance projects, SARS-CoV-2 variant testing, or future pandemics. This pipeline can be quickly established for further accreditation and clinical application.

Funder

National Institutes of Health

Publisher

MDPI AG

Subject

General Medicine

Link

https://www.mdpi.com/2673-8112/3/7/75/pdf

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