Detection of Neutralizing Antibodies in COVID-19 Patients from Steve Biko Academic Hospital Complex: A Pilot Study-Reference-Cited by-同舟云学术

Detection of Neutralizing Antibodies in COVID-19 Patients from Steve Biko Academic Hospital Complex: A Pilot Study

Published:2023-07-03 Issue:7 Volume:3 Page:999-1010
ISSN:2673-8112
Container-title:COVID
language:en
Short-container-title:COVID

Author:

Kgatle Mankgopo¹²,Chalwe Joseph Musonda³^ORCID,van der Westhuizen Donald⁴,Xu Shuting⁵,Damane Botle Precious⁶^ORCID,Mathebela Precious⁶^ORCID,Ueckermann Veronica⁷^ORCID,Mayaphi Simnikiwe⁸⁹^ORCID,Rodrigues Hosana Gomes¹⁰^ORCID,Moura-Alves Pedro¹¹¹²^ORCID,Ndlovu Honest¹²¹³,Mzizi Yonwaba¹²,Zongo Lusanda¹¹⁴^ORCID,Hairwadzi Henry¹⁵,Vorster Mariza¹²,Zeevaart Jan Rijn¹¹⁶^ORCID,Sathekge Mike¹²¹³^ORCID

Affiliation:

1. Nuclear Medicine Research Infrastructure (NuMeRI), Steve Biko Academic Hospital, Pretoria 0002, South Africa

2. Department of Nuclear Medicine, University of Pretoria & Steve Biko Academic Hospital, Pretoria 0001, South Africa

3. AXIM Nuclear and Oncology, Laboratory & Scientific Division, 63 Old Pretoria Road, Midrand 1685, South Africa

4. Inqaba Biotec, 525 Justice Mohamed Rd, Pretoria 0002, South Africa

5. GenScript Biotech (Netherlands) BV., 2288 EG Rijswijk, The Netherlands

6. Department of Surgery, University of Pretoria & Steve Biko Academic Hospital, Hatfield 0028, South Africa

7. Department Internal Medicine, University of Pretoria & Steve Biko Academic Hospital, Pretoria 0001, South Africa

8. Department of Medical Virology, University of Pretoria, Tshwane 0002, South Africa

9. National Health Laboratory Service-Tshwane Academic Division (NHLS-TAD), Tshwane 0002, South Africa

10. Laboratório de Nutrientes e Reparo Tecidual, Faculdade de Ciências Aplicadas, Universidade Estadual de Campinas, Limeira 13484-350, SP, Brazil

11. IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal

12. i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal

13. Department of Nuclear Medicine, Steve Biko Academic Hospital, Pretoria 0001, South Africa

14. Faculty of Health Science, University of Pretoria, Pretoria 0001, South Africa

15. Division of Hepatology and Liver Research, Department of Medicine, University of Cape Town, Groote Schuur Hospital, Cape Town 7925, South Africa

16. South African Nuclear Energy Corporation, Radiochemistry, Elias Motsoaledi Street, R104 Pelindaba, Northwest 0240, South Africa

Abstract

A correlation between neutralization activity after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination and protection against coronavirus disease 2019 (COVID-19) has been demonstrated by several studies. Here, we detect SARS-CoV-2 neutralizing antibody (NAB) production in COVID-19 patients from the Steve Biko Academic Hospital complex (SBAH), South Africa (SA). Samples from COVID-19 patients (mild to severe) were collected. SARS-CoV-2 rapid assays, genotyping (Delta and Omicron variants) and enzyme-linked immunosorbent assays (ELISA) were performed. IBM® Statistical Package for the Social Sciences (SPSS®) version 28 was used for inferential statistical analysis, and the data were presented using the Prism9 software (version 9.4.1). A total of 137 laboratory-confirmed COVID-19 patients, 12 vaccine recipients and 8 unvaccinated participants were evaluated. The production of SARS-CoV-2 NABs was observed in some of the COVID-19 cases, mainly in severe cases, although this should be noted with caution due to the small sample size of this pilot study. NABs were also observed in asymptomatic participants, with the most being found in recipients (n = 6) of the BNT162b2 (Pfizer-BioNTech) COVID-19 vaccine. We found a strong presence of NABs in COVID-19 patients, specifically in mild and severe cases. Severe infection was associated with higher NAB production (82%).

Funder

NUMERI

Publisher

MDPI AG

Subject

General Medicine

Link

https://www.mdpi.com/2673-8112/3/7/72/pdf

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