Effects of Oregano Essential Oil on IgA+, IgG+, and IgM+ Cells in the Jejunum of Castrated Holstein Bulls

Author:

Liu Qiyan1,Zhang Wangdong2,Wang Baoshan2,Shi Jinping1,He Pengjia1,Jia Li1,Huang Yongliang1ORCID,Xu Meiling1,Ma Yue1,Cheng Qiang3,Lei Zhaomin1

Affiliation:

1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

2. College of Animal Medicine, Gansu Agricultural University, Lanzhou 730070, China

3. Jing Chuan Xu Kang Food Co., Ltd., Pingliang 745000, China

Abstract

The aim of this study was to investigate the effect of oregano essential oil on IgA+, IgG+, and IgM+ cells in the jejunum of castrated Holstein bulls. Twelve castrated Holstein bulls were randomly divided into control (YCK) and oregano essential oil (YEO) groups. Pathological changes in the jejunum were observed by HE staining, and the expression levels of IgA, IgG, and IgM in the jejunum were detected by ELISA. The distributions of IgA+, IgG+, and IgM+ cells in the jejunum were analysed by multiplex immunofluorescence and immunohistochemistry. The results showed that the jejunal villi were detached in the YCK group, which may have been related to inflammation, while the intestinal epithelium was clear and intact in the YEO group. The expressions of IgA, IgG, and IgM were significantly reduced by 40.75%, 30.76%, and 50.87%. The IgA+, IgG+, and IgM+ cells were diffusely distributed in the lamina propria of the jejunum, and were reduced by 17.07%, 6.44%, and 6.15%, respectively. Oregano essential oil did not alter the distribution characteristics of IgA+, IgG+, or IgM+ cells in the jejunum, but it suppressed inflammatory response, decreased immunoglobulin content, and significantly enhanced the formation of an immune barrier in the gastrointestinal mucosa.

Funder

Gansu Provincial Education Science and Technology Innovation Project

Gansu Provincial Beef Cattle Quality Fattening Project

Gansu Provincial Agricultural Special Project

Publisher

MDPI AG

Subject

General Veterinary,Animal Science and Zoology

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