Acclimation and Blood Sampling: Effects on Stress Markers in C57Bl/6J Mice
Author:
Marin Nerea1, Moragon Amparo2, Gil Domingo2ORCID, Garcia-Garcia Francisco3, Bisbal Viviana1
Affiliation:
1. Animal Facility IISLAFE, Hospital La Fe Research Institute, 46026 Valencia, Spain 2. Animal Facility CIPF, Prince Felipe Research Center, 46012 Valencia, Spain 3. Bioinformatics & Biostatistics Unit CIPF, Prince Felipe Research Center, 46012 Valencia, Spain
Abstract
Blood sampling in rodents is common practice in scientific studies. Some of the refined methods widely used are the puncture of the saphenous vein or tail vein, or even tail docking. The handling needs of these different blood sampling methods are different and can directly affect stress, increasing the variability of the study. Moreover, there is less aversion and stress if the animal is accustomed to the environment, handling and technique. Therefore, our study aimed to assess the influence of these three blood sampling techniques (saphenous puncture, tail vein puncture and tail vein docking) and the use of previous acclimation on different indicators of animal stress, assessing blood glucose concentrations and faecal corticosterone metabolites (FCMs). Twenty-four young adult male and female C57Bl6/J mice were divided in three groups by sampling method: tail docking (TD), saphenous vein puncture (SV) and caudal vein puncture (CV) groups. All mice were studied with and without acclimation, which was performed during 9 consecutive days. The results showed that both males and females present very similar responses to the different handling and sampling methods without significant differences. Nevertheless, acclimation in all sampling methods decreased glucose and FCM levels significantly. The method that obtained the lowest glucose and FCM levels with significance was saphenous vein puncture. Therefore, we can say that it causes less stress when performing prior acclimation, even when this involves greater handling of the animal. Our results contribute to refinement within the 3R concept and could serve researchers to programme and select a good handling technique and a welfare-friendly blood sampling method for their experiments.
Subject
General Veterinary,Animal Science and Zoology
Reference44 articles.
1. Happy animals make good science;Poole;Lab. Anim.,1997 2. Improving quality of science through better animal welfare: The NC3Rs strategy;Prescott;Lab Anim.,2017 3. National Centre for the Replacement Refinement & Reduction of Animal in Research (NC3Rs) (2013). Blood Sampling Mouse, NC3Rs. 4. Removal of blood from laboratory mammals and birds. First report of the BVA/FRAME/RSPCA/UFAW Joint Working Group on Refinement;Morton;Lab. Anim.,1993 5. Dülsner, A., Hack, R., Krüger, C., Pils, M., Scherer, K., Schmelting, B., Schmidt, M., Weinert, H., and Jourdan, T. (2017). Recommendation for Blood Sampling in Laboratory Animals, Especially Small Laboratory Animals, Gesellschaft für Versuchstierkunde.
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