In Vivo Efficacy of Purified Quillaja Saponin Extracts in Protecting against Piscirickettsia salmonis Infections in Atlantic Salmon (Salmo salar)

Author:

Cortés Hernán1,Castillo-Ruiz Mario23ORCID,Cañon-Jones Hernán4ORCID,Schlotterbeck Trinidad1,San Martín Ricardo5,Padilla Leandro1

Affiliation:

1. Desert King Chile, Viña del Mar 2420505, Chile

2. Escuela de Química y Farmacia, Facultad de Medicina, Universidad Andres Bello, Santiago 8370134, Chile

3. Departamento de Ciencias Químicas y Biológicas, Facultad de Ciencias de la Salud, Universidad Bernardo O’Higgins, Santiago 8370854, Chile

4. Núcleo de Investigación Aplicada en Ciencias Veterinarias y Agronómicas, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Santiago 7500975, Chile

5. Sutardja Center for Entrepreneurship and Technology, College of Engineering, University of California, Berkeley, CA 94720, USA

Abstract

Piscirickettsiosis, the main infectious disease affecting salmon farming in Chile, still has no efficient control measures. Piscirickettsia salmonis is a facultative intracellular bacterium that can survive and replicate within the host macrophages, evading the immune response. Triterpenic saponins obtained from the Quillaja saponaria tree have been widely studied, and have been shown to be immunomodulatory agents, suitable for feed and vaccine applications for veterinary and human uses. The impact of the oral administration of two extracts of Quillaja saponins on the infection of P. salmonis in Salmo salar and the corresponding gene expressions of immunomarkers were studied under three in vivo models. In the intraperitoneal challenge model, the group fed with Quillaja extracts showed lower mortality (29.1% treated vs. 37.5% control). Similar results were obtained in the cohabitation model trial (36.3% vs. 60.0%). In the commercial pilot trial, the results showed a significant reduction of 71.3% in mortality caused by P. salmonis (0.51% vs. 1.78%) and antibiotic use (reduction of 66.6% compared to untreated control). Also, Quillaja extracts significantly modulated the expression of IFN-II and CD8. These results represent evidence supporting the future use of purified Quillaja extracts as a natural non-pharmacological strategy for the prevention and control of P. salmonis infections in salmon.

Funder

CONICYT PAI Project

CORFO Validation and Packaging of Innovatio, “Strategic Programs” by the Government of Chile

Publisher

MDPI AG

Subject

General Veterinary,Animal Science and Zoology

Reference47 articles.

1. Piscirickettsiosis and Piscirickettsia Salmonis in Fish: A Review;Rozas;J. Fish Dis.,2014

2. Piscirickettsia Salmonis: A Gram-Negative Intracellular Bacterial Pathogen of Fish;Fryer;J. Fish Dis.,2003

3. Departamento de Salud Animal (2022). Informe Sanitario Con Información Sanitaria de Agua Dulce Y Mar 1° Semestre Año 2022 Departamento de Salud Animal Subdirección de Acuicultura Servicio Nacional de Pesca Y Acuicultura, Departamento de Salud Animal.

4. Watts, J.E.M., Schreier, H.J., Lanska, L., and Hale, M.S. (2017). The Rising Tide of Antimicrobial Resistance in Aquaculture: Sources, Sinks and Solutions. Mar. Drugs, 15.

5. Current Status of the Use of Antibiotics and the Antimicrobial Resistance in the Chilean Salmon Farms;Miranda;Fron. Microbiol.,2018

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3