Edwardsiella tarda in Tambaqui (Colossoma macropomum): A Pathogenicity, Antimicrobial Susceptibility, and Genetic Analysis of Brazilian Isolates
Author:
Reis Francisco Yan Tavares1ORCID, Rocha Victória Pontes1, Janampa-Sarmiento Peter Charrie1ORCID, Costa Henrique Lopes1, Egger Renata Catão1ORCID, Passos Naísa Cristine1, de Assis Carlos Henrique Santos1, Carneiro Sarah Portes1, Santos Ágna Ferreira2, Silva Brendhal Almeida1, Dorella Fernanda Alves1, Leibowitz Márcia Pimenta1ORCID, Luz Ronald Kennedy3ORCID, Pierezan Felipe2, Gallani Sílvia Umeda4, Tavares Guilherme Campos1ORCID, Figueiredo Henrique César Pereira1ORCID
Affiliation:
1. Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil 2. Department of Veterinary Clinics and Surgery, School of Veterinary Medicine, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil 3. Department of Animal Science, School of Veterinary Medicine, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil 4. Postgraduate Program in Aquaculture, Nilton Lins University, Manaus 69058-030, AM, Brazil
Abstract
Edwardsiella tarda is a crucial pathogenic bacterium in tropical aquaculture. This bacterium was recently isolated from tambaqui (Colossoma macropomum), a commercially important fish species in Brazil. This study assessed the antimicrobial susceptibility, pathogenicity, and genetic diversity of the tambaqui-derived E. tarda isolates. Fourteen bacterial isolates isolated from tambaqui were identified as E. tarda by using matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry and dnaJ gene sequencing. Antimicrobial susceptibility tests were conducted against seven drugs using the disc diffusion assay. The pathogenicity test conducted by intraperitoneal injection of 2.4 × 107 colony-forming units (CFU) fish−1 of E. tarda (ED38-17) into tambaqui juveniles eventually revealed that neither clinical signs nor death were present. However, splenomegaly and whitish areas in the spleen and kidneys were observed. The histological investigation also revealed granulomatous splenitis, nephritis, and hepatitis occurring internally. Repetitive extragenic palindromic-PCR fingerprinting separated the 14 isolates into three genetic groups. The antibiogram revealed that all E. tarda isolates were wild-type (WT) to florfenicol (FLO), norfloxacin (NOR), neomycin (NEO), erythromycin (ERY), and oxytetracycline (OXY); however, some were non-wild-type to sulfamethoxazole/trimethoprim (7.1%) and amoxicillin (21.4%). Therefore, through experimental infection, E. tarda ED38-17 could induce pathogenic effects in C. macropomum. Additionally, three distinct genetic types were found, and the E. tarda isolates were WT to FLO, NOR, NEO, ERY, and OXY. These findings raise awareness of a bacteria causing unseen lesions, a pathogen that will potentially impact tambaqui aquaculture in the future.
Funder
Coordination for the Improvement of Higher Education Personnel—Brazil (CAPES) through the National Academic Cooperation Program in the Amazon–PROCAD/Amazônia Conselho Nacional de Desenvolvimento Científico e Tecnológico Fundação de Amparo à Pesquisa do Estado de Minas Gerais CNPq
Subject
General Veterinary,Animal Science and Zoology
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