Author:
Grosch Johannes,Eberlein Bernadette,Waldherr Sebastian,Pascal Mariona,San Bartolomé Clara,De La Roca Pinzón Federico,Dittmar Michael,Hilger Christiane,Ollert Markus,Biedermann Tilo,Darsow Ulf,Bilò Maria Beatrice,Schmidt-Weber Carsten B.,Blank Simon
Abstract
Discriminating Polistes dominula and Vespula spp. venom allergy is of growing importance worldwide, as systemic reactions to either species’ sting can lead to severe outcomes. Administering the correct allergen-specific immunotherapy is therefore a prerequisite to ensure the safety and health of venom-allergic patients. Component-resolved diagnostics of Hymenoptera venom allergy might be improved by adding additional allergens to the diagnostic allergen panel. Therefore, three potential new allergens from P. dominula venom—immune responsive protein 30 (IRP30), vascular endothelial growth factor C (VEGF C) and phospholipase A2 (PLA2)—were cloned, recombinantly produced and biochemically characterized. Sera sIgE titers of Hymenoptera venom-allergic patients were measured in vitro to assess the allergenicity and potential cross-reactivity of the venom proteins. IRP30 and VEGF C were classified as minor allergens, as sensitization rates lay around 20–40%. About 50% of P. dominula venom-allergic patients had measurable sIgE titers directed against PLA2 from P. dominula venom. Interestingly, PLA2 was unable to activate basophils of allergic patients, questioning its role in the context of clinically relevant sensitization. Although the obtained results hint to a questionable benefit of the characterized P. dominula venom proteins for improved diagnosis of venom-allergic patients, they can contribute to a deeper understanding of the molecular mechanisms of Hymenoptera venoms and to the identification of factors that determine the allergenic potential of proteins.
Subject
Health, Toxicology and Mutagenesis,Toxicology
Reference54 articles.
1. Polistes dominulus (Hymenoptera, Vespidae) invading North America: some hypotheses for its rapid spread
2. Polistes dominulus(Christ, 1791) (Hymenoptera: Polistinae: Vespidae) Newly Recorded from South Africa
3. Notes on the occurrence of Vespidae (Hym.) in Mecklenburg, mainly around Rostock. (Zum Vorkommen von Faltenwespen (Hym., Vespidae) in Mecklenburg mit Schwerpunkt im Raum Rostock.);Duty;Entomol. Nachr. Ber.,1997
4. Remarks on the taxonomy and distribution of two species of paper wasps—Polistes gallicus (Linnaeus, 1767) and P. dominulus (Christ, 1791) (Hymenoptera: Vespidae) in Poland;Kowalczyk;Wiadomości Entomol.,2003
5. Self-organization of populations of polistine wasps (Hymenoptera, Vespidae, Polistinae)
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献