Abstract
Adsorbents for whole blood apheresis need to be highly blood compatible to minimize the activation of blood cells on the biomaterial surface. Here, we developed blood-compatible matrices by surface modification with polyzwitterionic polysulfobetainic and polycarboxybetainic coatings. Photoreactive zwitterionic terpolymers were synthesized by free-radical polymerization of zwitterionic, photoreactive, and fluorescent monomers. Upon UV irradiation, the terpolymers were photodeposited and mutually crosslinked on the surface of hydrophobic polystyrene-co-divinylbenzene and hydrophilic polyacrylamide-co-polyacrylate (DALI) beads. Fluorescent microscopy revealed coatings with an average thickness of 5 µm, which were limited to the bead surface. Blood compatibility was assessed based on polymer-induced hemolysis, coagulation parameters, and in vitro tests. The maintenance of the adsorption capacity after coating was studied in human whole blood with cytokines for polystyrene beads (remained capacity 25–67%) and with low-density lipoprotein (remained capacity 80%) for polyacrylate beads. Coating enhanced the blood compatibility of hydrophobic, but not of hydrophilic adsorbents. The most prominent effect was observed on coagulation parameters (e.g., PT, aPTT, TT, and protein C) and neutrophil count. Polycarboxybetaine with a charge spacer of five carbons was the most promising polyzwitterion for the coating of adsorbents for whole blood apheresis.
Funder
country of Lower Austria
Christian Doppler Society
Subject
Biomedical Engineering,Biomaterials
Reference80 articles.
1. Long-Term Lipoprotein Apheresis in the Treatment of Severe Familial Hypercholesterolemia Refractory to High Intensity Statin Therapy: Three Year Experience at a Lipoprotein Apheresis Center;Cardiol. J.,2019
2. Apheresis for Severe Hypercholesterolaemia and Elevated Lipoprotein(A);Pathology,2019
3. Therapeutic Apheresis for Management of Lp(a) Hyperlipoproteinemia;Curr. Atheroscler. Rep.,2020
4. Extracorporeal Techniques for the Treatment of Critically Ill Patients with Sepsis beyond Conventional Blood Purification Therapy: The Promises and the Pitfalls 11 Medical and Health Sciences 1107 Immunology;Crit. Care,2018
5. Clinical Utility of Extracorporeal Cytokine Hemoadsorption Therapy: A Literature Review;Blood Purif.,2018
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