Abstract
Gas plasma is a medical technology with antimicrobial properties. Its main mode of action is oxidative damage via reactive species production. The clinical efficacy of gas plasma-reduced bacterial burden has been shown to be hampered in some cases. Since the reactive species profile produced by gas plasma jets, such as the kINPen used in this study, are thought to determine antimicrobial efficacy, we screened an array of feed gas settings in different types of bacteria. Antimicrobial analysis was performed by single-cell analysis using flow cytometry. We identified humidified feed gas to mediate significantly greater toxicity compared to dry argon and many other gas plasma conditions. The results were confirmed by inhibition zone analysis on gas-plasma-treated microbial lawns grown on agar plates. Our results may have vital implications for clinical wound management and potentially enhance antimicrobial efficacy of medical gas plasma therapy in patient treatment.
Funder
Federal Ministry of Education and Research
Subject
Paleontology,Space and Planetary Science,General Biochemistry, Genetics and Molecular Biology,Ecology, Evolution, Behavior and Systematics
Reference42 articles.
1. Treatment of Chronic Infection;Cierny;J. Am. Acad. Orthop. Surg.,2006
2. Biofilms and Their Potential Role in Wound Healing;Percival;Wounds-A Compend. Clin. Res. Pract.,2004
3. Chronic Wound Repair and Healing in Older Adults: Current Status and Future Research;Gould;J. Am. Geriatr. Soc.,2015
4. Management of Venous Ulcers: State of the Art;Marola;Int. J. Surg.,2016
5. Advanced Therapeutic Dressings for Effective Wound Healing—A Review;Boateng;J. Pharm. Sci.,2015
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献