The Modification of Cell Wall Properties Is Involved in the Growth Inhibition of Rice Coleoptiles Induced by Lead Stress

Abstract

Lead (Pb) is a widespread heavy metal pollutant that interferes with plant growth. In this study, we investigated the effects of Pb on the mechanical and chemical properties of cell walls and on the growth of coleoptiles of rice (Oryza sativa L.) seedlings grown in the air (on moistened filter paper) and underwater (submerged condition). Coleoptile growth of air-grown seedlings was reduced by 40% by the 3 mM Pb treatment, while that of water-grown ones was reduced by 50% by the 0.5 mM Pb. Although the effective concentration of Pb for growth inhibition of air-grown coleoptiles was much higher than that of water-grown ones, Pb treatment significantly decreased the mechanical extensibility of the cell wall in air- and water-grown coleoptiles, when it inhibited their growth. Among the chemical components of coleoptile cell walls, the amounts of cell wall polysaccharides per unit fresh weight and unit length of coleoptile, which represent the thickness of the cell wall, were significantly increased in response to the Pb treatment (3 mM and 0.5 mM Pb for air- and water-grown seedlings, respectively), while the levels of cell wall-bound diferulic acids (DFAs) and ferulic acids (FAs) slightly decreased. These results indicate that Pb treatment increased the thickness of the cell wall but not the phenolic acid-mediated cross-linking structures within the cell wall in air- and water-grown coleoptiles. The Pb-induced cell wall thickening probably causes the mechanical stiffening of the cell wall and thus decreases cell wall extensibility. Such modifications of cell wall properties may be associated with the inhibition of coleoptile growth. The results of this study provide a new finding that Pb-induced cell wall remodeling contributes to the regulation of plant growth under Pb stress conditions via the modification of the mechanical property of the cell wall.