Genetic Evaluation of Juniperus sabina L. (Cupressaceae) in Arid and Semi-Arid Regions of China Based on SSR Markers

Abstract

Juniperus sabina L., a shrub distributed in patches in arid and semi-arid areas of the northern hemisphere, plays an important role in preventing land desertification and maintaining ecosystems. However, few studies have reported genetic diversity and genetic structure of widely distributed populations of J. sabina in northwest China. Here, we evaluated the genetic diversity and genetic structure and predicted the isolation barriers among 11 populations based on 20 simple sequence repeats (SSRs). A total of 134 alleles were generated and the average number of alleles per locus was 6.70. The Shannon diversity index ranged from 0.659 to 0.951, with an average of 0.825. Population structure analysis revealed that the populations were assigned into two genetic groups. The analysis of molecular variance (AMOVA) indicated that 88% of genetic variation existed within populations. Moderate population differentiation was occurred with FST value of 0.090. Finally, we concluded that geographic isolation is the main factor affecting the genetic structure of J. sabina populations. The results of this study provide a foundation for the strategies for J. sabina genetic conservation and management.