Affiliation:
1. College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China
2. Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China
3. Collaborative Innovation Center for Water Pollution Control and Water Security in Karst Region, Guilin University of Technology, Guilin 541004, China
Abstract
In recent years, β-Hexachlorocyclohexane (β-HCH) has been detected frequently in water, seriously threatening human health and ecological balance. To explore the effects of different treatment groups on the removal of β-HCH in experimental water and the response of microbial community structure in the system, three strains of β-HCH-degrading bacteria—Ochrobactrum sp. (Och1, Och2) and Pseudomonas sp. (Pse1)—combined with Canna were selected for microbial, plant, and microbe–plant repair hydroponic experiments, respectively. Solid-phase extraction combined with GC-ECD and high-throughput sequencing determined the β-HCH content and bacterial community in water and Canna tissues. The results showed that when β-HCH stress concentrations were 10 μg·L−1 and 100 μg·L−1, Och1 and Pse1 showed the best degradation performance (33.49% and 60.02%, respectively). Following this, the three degrading strains were combined with Canna. Under the two β-HCH stress concentrations, the combination of Och1–Canna showed the highest β-HCH removal efficiency (96.74% and 99.06%). At the same time, we measured the concentration of β-HCH in Canna tissues and found that Och1 had a better removal effect on β-HCH in water and that the addition of Pse1 may significantly improve the absorption capacity of β-HCH in Canna roots. In addition, the relative abundance of Methophilic bacteria in experimental water and Canna root samples increased significantly after the inoculation of degrading bacteria, suggesting that Methophilic bacteria may be vital in degrading benzene-ring-containing substances. The results of this research can provide a theoretical basis and technical support for the prevention and control of the non-point source pollution of organic pesticides.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Guangxi Province, China
Guilin University of Technology, China
Subject
Water Science and Technology,Aquatic Science,Geography, Planning and Development,Biochemistry