Affiliation:
1. Laboratório de Mosquitos Transmissores de Hematozoários, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, RJ, Brazil
2. Department of Arbovirology, Bernhard Nocht Institute of Tropical Medicine, 20359 Hamburg, Germany
Abstract
Background: The mosquito microbiota impacts different parameters in host biology, such as development, metabolism, immune response and vector competence to pathogens. As the environment is an important source of acquisition of host associate microbes, we described the microbiota and the vector competence to Zika virus (ZIKV) of Aedes albopictus from three areas with distinct landscapes. Methods: Adult females were collected during two different seasons, while eggs were used to rear F1 colonies. Midgut bacterial communities were described in field and F1 mosquitoes as well as in insects from a laboratory colony (>30 generations, LAB) using 16S rRNA gene sequencing. F1 mosquitoes were infected with ZIKV to determine virus infection rates (IRs) and dissemination rates (DRs). Collection season significantly affected the bacterial microbiota diversity and composition, e.g., diversity levels decreased from the wet to the dry season. Field-collected and LAB mosquitoes’ microbiota had similar diversity levels, which were higher compared to F1 mosquitoes. However, the gut microbiota composition of field mosquitoes was distinct from that of laboratory-reared mosquitoes (LAB and F1), regardless of the collection season and location. A possible negative correlation was detected between Acetobacteraceae and Wolbachia, with the former dominating the gut microbiota of F1 Ae. albopictus, while the latter was absent/undetectable. Furthermore, we detected significant differences in infection and dissemination rates (but not in the viral load) between the mosquito populations, but it does not seem to be related to gut microbiota composition, as it was similar between F1 mosquitoes regardless of their population. Conclusions: Our results indicate that the environment and the collection season play a significant role in shaping mosquitoes’ bacterial microbiota.
Funder
Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES), Brazil
Edital Universal CNPq, 2018
Inova Fiocruz/Fundação Oswaldo Cruz, Edital Geração de Conhecimento—Novos Talentos 2018
Subject
Virology,Infectious Diseases
Reference127 articles.
1. Harbach, R.E. (2022, September 06). Mosquito Taxonomic Inventory. Available online: https://mosquito-taxonomic-inventory.myspecies.info.
2. Present and Future Arboviral Threats;Weaver;Antivir. Res.,2010
3. Dengue Fever in Mainland China;Wu;Am. J. Trop. Med. Hyg.,2010
4. First Autochthonous Dengue Outbreak in Italy, August 2020;Lazzarini;Eurosurveillance,2020
5. Characterization of the First Autochthonous Dengue Outbreak in Spain (August–September 2018);Monge;Acta Trop.,2020
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