Inclusion Bodies in Ionic Liquids-Reference-Cited by-同舟云学术

Inclusion Bodies in Ionic Liquids

Published:2023-12-22 Issue:1 Volume:4 Page:1-31
ISSN:2673-8015
Container-title:Liquids
language:en
Short-container-title:Liquids

Author:

Szabadi András¹²^ORCID,Klausser Robert³⁴^ORCID,Spadiut Oliver³⁴^ORCID,Schröder Christian¹^ORCID

Affiliation:

1. Faculty of Chemistry, Institute for Computational Biological Chemistry, University of Vienna, Währingerstr. 17, 1090 Vienna, Austria

2. Doctoral School in Chemistry (DoSChem), University of Vienna, Währingerstr. 42, 1090 Vienna, Austria

3. Integrated Bioprocess Development, Technische Universität Wien, Gumpendorferstrasse 1A/5th Floor, 1060 Vienna, Austria

4. Christian Doppler Laboratory IB Processing 4.0, Technische Universität Wien, Gumpendorferstrasse 1A/5th Floor, 1060 Vienna, Austria

Abstract

The pivotal role of proteins in pharmaceuticals is challenged by stability issues, making the study of inclusion bodies—a source of insoluble protein aggregates—increasingly relevant. This review outlines the critical procedures in inclusion body processing, focusing on ’mild solubilization concepts’ and refolding methodologies. Attention is afforded to the emerging role of ionic liquids with unique and tunable physicochemical properties in optimizing protein unfolding and refolding processes. The review critically assesses the existing literature at the intersection of inclusion bodies and ionic liquids, identifying recent advancements, potential applications, and avenues for future research. This comprehensive analysis aims to elucidate the complexities in efficient protein processing from inclusion bodies.

Funder

Christian Doppler Research Association

Publisher

MDPI AG

Subject

General Medicine

Link

https://www.mdpi.com/2673-8015/4/1/1/pdf

Reference177 articles.

1. Klausser, R., Kopp, J., Brichtova, E.P., Gisperg, F., Elshazly, M., and Spadiut, O. (2023). State-of-the-art and novel approaches to mild solubilization of inclusion bodies. Front. Bioeng. Biotechnol., 11.

2. Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine;Tsumoto;Biochem. Biophys. Res. Commun.,2003

3. Nondenaturing solubilization of β2 microglobulin from inclusion bodies by L-arginine;Umetsu;Biochem. Biophys. Res. Commun.,2005

4. Bioprocessing of recombinant proteins from Escherichia coli inclusion bodies: Insights from structure-function relationship for novel applications;Kachhawaha;Prep. Biochem. Biotechnol.,2022

5. Buscajoni, L., Martinetz, M.C., Berkemeyer, M., and Brocard, C. (2022). Refolding in the modern biopharmaceutical industry. Biotechnol. Adv., 61.