Affiliation:
1. Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, SC 29634, USA
2. Clemson Light Imaging Facility, Clemson University, Clemson, SC 29634, USA
Abstract
Obesity is a condition caused by surplus adipose tissue and is a risk factor for several diet-related diseases. Obesity is a global epidemic that has also been challenging to treat effectively. However, one promoted therapy to safely treat obesity is anti-adipogenic therapeutics. Therefore, identifying potent anti-adipogenic bioactive compounds that can safely be used clinically may effectively treat obesity in humans. Mango leaf has potential medicinal properties due to its many bioactive compounds that may enhance human health. Mangiferin (MGF) is a primary constituent in mango plants, with many health-promoting qualities. Therefore, this study investigated the effect of MGF, and tea brewed with mango leaves in cultured adipocytes. The anti-adipogenic efficacy of mango leaf tea (MLT) and MGF in 3T3-L1 cells were assessed, along with cell viability, triglyceride levels, adiponectin secretion, and glucose uptake analyzed. In addition, changes in the mRNA expression of genes involved in lipid metabolism within 3T3-L1 cells were determined using quantitative real-time PCR. Our results showed while both MLT and MGF increased glucose uptake in adipocytes, only MLT appeared to inhibit adipogenesis, as determined by decreased triglyceride accumulation. We also observed increased secretory adiponectin levels, reduced ACC mRNA expression, and increased FOXO1 and ATGL gene expression in 3T3-L1 cells treated with MLT but not MGF. Together, these results suggest that MLT may exhibit anti-adipogenic properties independent of MGF content.
Funder
NIH Eukaryotic Pathogens Innovation Center, Centers of Biomedical Research Excellence
NIH SC BioCRAFT Centers of Biomedical Research Excellence
Clemson University Division of Research
Reference48 articles.
1. Diabetes and cancer: Two diseases with obesity as a common risk factor;Garg;Diabetes Obes. Metab.,2014
2. Fucoxanthin exerts differing effects on 3T3-L1 cells according to the differentiation stage and inhibits glucose uptake in mature adipocytes;Kang;Biochem. Biophys. Res. Commun.,2011
3. Obesity in the pathogenesis of type 2 diabetes;Day;Br. J. Diabetes Vasc. Dis.,2011
4. Epidemiology of obesity and diabetes and their cardiovascular complications;Bhupathiraju;Circ. Res.,2016
5. Park, E., Kim, J., Yeo, S., Kim, G., Ko, E., Lee, S.W., Li, W.Y., Choi, C.W., and Jeong, S. (2018). Antiadipogenic effects of loganic acid in 3T3-L1 preadipocytes and ovariectomized mice. Molecules, 23.