Effect of Superovulation Treatment on Oocyte’s DNA Methylation

Author:

Lopes Jordana S.ORCID,Ivanova Elena,Ruiz SalvadorORCID,Andrews SimonORCID,Kelsey GavinORCID,Coy PilarORCID

Abstract

Controlled ovarian stimulation is a necessary step in some assisted reproductive procedures allowing a higher collection of female gametes. However, consequences of this stimulation for the gamete or the offspring have been shown in several mammals. Most studies used comparisons between oocytes from different donors, which may contribute to different responses. In this work, we use the bovine model in which each animal serves as its own control. DNA methylation profiles were obtained by single-cell whole-genome bisulfite sequencing of oocytes from pre-ovulatory unstimulated follicles compared to oocytes from stimulated follicles. Results show that the global percentage of methylation was similar between groups, but the percentage of methylation was lower for non-stimulated oocytes in the imprinted genes APEG3, MEG3, and MEG9 and higher in TSSC4 when compared to stimulated oocytes. Differences were also found in CGI of imprinted genes: higher methylation was found among non-stimulated oocytes in MEST (PEG1), IGF2R, GNAS (SCG6), KvDMR1 ICR UMD, and IGF2. In another region around IGF2, the methylation percentage was lower for non-stimulated oocytes when compared to stimulated oocytes. Data drawn from this study might help to understand the molecular reasons for the appearance of certain syndromes in assisted reproductive technologies-derived offspring.

Funder

European Union Horizon 2020 Marie Skłodowska-Curie Action

Spanish Ministry of Economy and Competitiveness (MINECO) & European Regional Development Fund

Spanish Ministry of Science and Innovation

Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia

the UK Biotechnology and Biological Sciences Research Council

Medical Research Council

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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