Nanostructured Coatings Based on Graphene Oxide for the Management of Periprosthetic Infections-Reference-Cited by-同舟云学术

Nanostructured Coatings Based on Graphene Oxide for the Management of Periprosthetic Infections

Published:2024-02-17 Issue:4 Volume:25 Page:2389
ISSN:1422-0067
Container-title:International Journal of Molecular Sciences
language:en
Short-container-title:IJMS

Author:

Constantinescu Sorin¹,Niculescu Adelina-Gabriela²³^ORCID,Hudiță Ariana²⁴^ORCID,Grumezescu Valentina⁵,Rădulescu Dragoș¹,Bîrcă Alexandra Cătălina³,Dorcioman Gabriela⁵^ORCID,Gherasim Oana⁵,Holban Alina Maria²⁶,Gălățeanu Bianca⁴^ORCID,Vasile Bogdan Ștefan³^ORCID,Grumezescu Alexandru Mihai²³^ORCID,Bolocan Alexandra¹,Rădulescu Radu¹

Affiliation:

1. Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari Street, 050474 Bucharest, Romania

2. Research Institute of the University of Bucharest—ICUB, University of Bucharest, 90-92 Panduri, 050663 Bucharest, Romania

3. Department of Science and Engineering of Oxide Materials and Nanomaterials, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 060042 Bucharest, Romania

4. Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei Street, 050095 Bucharest, Romania

5. Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, Romania

6. Microbiology and Immunology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Lane, 77206 Bucharest, Romania

Abstract

To modulate the bioactivity and boost the therapeutic outcome of implantable metallic devices, biodegradable coatings based on polylactide (PLA) and graphene oxide nanosheets (nGOs) loaded with Zinforo™ (Zin) have been proposed in this study as innovative alternatives for the local management of biofilm-associated periprosthetic infections. Using a modified Hummers protocol, high-purity and ultra-thin nGOs have been obtained, as evidenced by X-ray diffraction (XRD) and transmission electron microscopy (TEM) investigations. The matrix-assisted pulsed laser evaporation (MAPLE) technique has been successfully employed to obtain the PLA-nGO-Zin coatings. The stoichiometric and uniform transfer was revealed by infrared microscopy (IRM) and scanning electron microscopy (SEM) studies. In vitro evaluation, performed on fresh blood samples, has shown the excellent hemocompatibility of PLA-nGO-Zin-coated samples (with a hemolytic index of 1.15%), together with their anti-inflammatory ability. Moreover, the PLA-nGO-Zin coatings significantly inhibited the development of mature bacterial biofilms, inducing important anti-biofilm efficiency in the as-coated samples. The herein-reported results evidence the promising potential of PLA-nGO-Zin coatings to be used for the biocompatible and antimicrobial surface modification of metallic implants.

Publisher

MDPI AG

Link

https://www.mdpi.com/1422-0067/25/4/2389/pdf

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