Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (Cucurbita pepo) Lines-Reference-Cited by-同舟云学术

Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (Cucurbita pepo) Lines

Published:2023-03-19 Issue:3 Volume:15 Page:788
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Kavalappara Saritha Raman¹,Bag Sudeep¹^ORCID,Luckew Alex²,McGregor Cecilia E.²

Affiliation:

1. Department of Plant Pathology, University of Georgia, Tifton, GA 31793, USA

2. Department of Horticulture, University of Georgia, Athens, GA 30602, USA

Abstract

RNA silencing is a crucial mechanism of the antiviral immunity system in plants. Small RNAs guide Argonaut proteins to target viral RNA or DNA, preventing virus accumulation. Small RNA profiles in Cucurbita pepo line PI 420328 with tolerance to cucurbit yellow stunting disorder virus (CYSDV) were compared with those in Gold Star, a susceptible cultivar. The lower CYSDV symptom severity in PI 420328 correlated with lower virus titers and fewer sRNAs derived from CYSDV (vsRNA) compared to Gold Star. Elevated levels of 21- and 22-nucleotide (nt) size class vsRNAs were observed in PI 420328, indicating more robust and efficient RNA silencing in PI 420328. The distribution of vsRNA hotspots along the CYSDV genome was similar in both PI 420328 and Gold Star. However, the 3’ UTRs, CPm, and p26 were targeted at a higher frequency in PI 420328.

Funder

USDA-UGA Cooperative Agreement

USDA-National Institute of Food and Agriculture-HATCH project

Publisher

MDPI AG

Subject

Virology,Infectious Diseases

Link

https://www.mdpi.com/1999-4915/15/3/788/pdf

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