Affiliation:
1. Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Republic of Korea
Abstract
Acinetobacter baumannii is a critically hard-to-treat gram-negative pathogen responsible for a range of infectious diseases. Tigecycline is a last-resort antibiotic for A. baumannii infection; however, tigecycline-resistant (TIG-R) A. baumannii has been increasingly reported. Therefore, new strategies must be developed to treat these detrimental infections. Nanoantibiotics composed of two-dimensional (2D) black phosphorus (BP) and its derived nanocomposites have emerged as excellent alternatives to current antibiotics. However, the development of unique materials to target specific pathogens is challenging. Here, we report the preparation of a BP-based ZnO-Ag (ZPBA) nanocomposite. A low-temperature solution synthesis method was used to prepare ZnO and Ag nanoparticles immobilized on BP nanosheets. X-ray diffraction, transmission electron microscopy, and X-ray photoelectron spectroscopy were used to characterize the ZPBA nanocomposite. The antibacterial activity of ZPBA nanocomposite was assessed by determining its minimum inhibitory concentration against type (ATCC 19606, ATCC 15150) and TIG-R (ATCC 19606-R) A. baumannii strains. From the assays, ZPBA showed superior activity against TIG-R A. baumannii strain with MIC of 12.5 µg·mL−1 compared to all other prepared samples. Finally, the combination of bacterial membrane disruption and ROS generation was demonstrated to be a potential antibacterial mechanism of ZPBA. Our results show that ZPBA could be a potential nanoantibiotic platform for eradicating TIG-R A. baumannii.
Funder
National Research Foundation of Korea
Pusan National University
Subject
Engineering (miscellaneous),Ceramics and Composites
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