Boosting PRRSV-Specific Cellular Immunity: The Immunological Profiling of an Fc-Fused Multi-CTL Epitope Vaccine in Mice-Reference-Cited by-同舟云学术

Boosting PRRSV-Specific Cellular Immunity: The Immunological Profiling of an Fc-Fused Multi-CTL Epitope Vaccine in Mice

Published:2024-06-15 Issue:6 Volume:11 Page:274
ISSN:2306-7381
Container-title:Veterinary Sciences
language:en
Short-container-title:Veterinary Sciences

Author:

Lei Xinnuo¹^ORCID,Ban Jinzhao¹²,Wu Zhi¹,Cao Shinuo¹,Zhou Mo¹,Zhang Li¹,Zhu Rui¹,Lu Huipeng¹,Zhu Shanyuan¹

Affiliation:

1. Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Engineering Technology Research Center for Modern Animal Science and Novel Veterinary Pharmaceutic Development, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China

2. Ministry of Agriculture Key Laboratory of Animal Bacteriology, International Joint Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

Abstract

The continuously evolving PRRSV has been plaguing pig farms worldwide for over 30 years, with conventional vaccines suffering from insufficient protection and biosecurity risks. To address these challenges, we identified 10 PRRSV-specific CTL epitopes through enzyme-linked immunospot assay (ELISPOT) and constructed a multi-epitope peptide (PTE) by linking them in tandem. This PTE was then fused with a modified porcine Fc molecule to create the recombinant protein pFc-PTE. Our findings indicate that pFc-PTE effectively stimulates PRRSV-infected specific splenic lymphocytes to secrete high levels of interferon-gamma (IFN-γ) and is predicted to be non-toxic and non-allergenic. Compared to PTE alone, pFc-PTE not only induced a comparable cellular immune response in mice but also extended the duration of the immune response to at least 10 weeks post-immunization. Additionally, pFc-PTE predominantly induced a Th1 immune response, suggesting its potential advantage in enhancing cellular immunity. Consequently, pFc-PTE holds promise as a novel, safe, and potent candidate vaccine for PRRSV and may also provide new perspectives for vaccine design against other viral diseases.

Funder

Jiangsu Agri-animal Husbandry Vocational College

Publisher

MDPI AG

Link

https://www.mdpi.com/2306-7381/11/6/274/pdf

Reference35 articles.

1. Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Pathogenesis and Interaction with the Immune System;Lunney;Annu. Rev. Anim. Biosci.,2016

2. Zhao, H.Z., Wang, F.X., Han, X.Y., Guo, H., Liu, C.Y., Hou, L.N., Wang, Y.X., Zheng, H., Wang, L., and Wen, Y.J. (2022). Recent advances in the study of NADC34-like porcine reproductive and respiratory syndrome virus in China. Front. Microbiol., 13.

3. Ruedas-Torres, I., Rodríguez-Gómez, I.M., Sánchez-Carvajal, J.M., Larenas-Muñoz, F., Pallarés, F.J., Carrasco, L., and Gómez-Laguna, J. (2021). The jigsaw of PRRSV virulence. Vet. Microbiol., 260.

4. The ins and outs of MHC class II-mediated antigen processing and presentation;Roche;Nat. Rev. Immunol.,2015

5. Wang, J., Zhang, M., Cui, X., Gao, X., Sun, W., Ge, X., Zhang, Y., Guo, X., Han, J., and Zhou, L. (2022). Attenuated Porcine Reproductive and Respiratory Syndrome Virus Regains Its Fatal Virulence by Serial Passaging in Pigs or Porcine Alveolar Macrophages To Increase Its Adaptation to Target Cells. Microbiol. Spectr., 10.