Variation in Interleukin-4, -6, and -10 in Mastitis Milk: Associations with Infections, Pathogens, Somatic Cell Counts, and Oxidative Stress

Author:

Chaisri Wasana12,Intanon Montira12,Saipinta Duanghathai12,Srithanasuwan Anyaphat3,Pangprasit Noppason4ORCID,Jaraja Weerin1,Chuasakhonwilai Areerat1,Suriyasathaporn Witaya125

Affiliation:

1. Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand

2. Research Center of Producing and Development of Products and Innovations for Animal Health and Production, Chiang Mai University, Chiang Mai 50100, Thailand

3. Department of Animal Sciences, Wageningen University, 6700 AH Wageningen, The Netherlands

4. Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand

5. Cambodia Campus, Asian Satellite Campuses Institute, Nagoya University, Nagoya 464-8601, Japan

Abstract

Poor mastitis control favors intramammary infection (IMI), which always involves CNS. This study aimed to determine the relationships of IL-4, IL-6, and IL-10 in mastitis milk with concurrent infection, bacterial pathogens, SCC, and MDA, an oxidative stress marker. All mastitis quarters from five smallholder dairy farms were sampled aseptically before morning milking and again before afternoon milking for bacteriological identification using MALDI-TOF mass spectrometry. The samples with the concomitant infection between streptococci and CNS and their pairs of another sample from the quarters were selected. In addition, samples were randomly chosen to have a controlled single infection. IL-4, IL-6, and IL-10 were measured with ELISA kits. MDA was measured using HPLC, while SCC was measured using Fossomatic™ FC. The results from a repeated measure analysis showed that IL-4 positively correlated with SCC, while IL-6 showed a negative trend. IL-4 levels were highest in CNS infections and significantly higher than in non-infected or mixed infections (p < 0.05). The IL-6 level of the mixed bacteria was highest and showed a different trend from non-infection, and the quarter was infected with streptococcal bacteria. In conclusion, from a single infection, the streptococci and CNS quarter showed varied immune responses, including trendily higher IL-6 and IL-4.

Funder

Thailand Science Research and Innovation

Publisher

MDPI AG

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